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Publicação:
Cissus quadrangularis Linn exerts dose-dependent biphasic effects: osteogenic and anti-proliferative, through modulating ROS, cell cycle and Runx2 gene expression in primary rat osteoblasts

dc.contributor.authorSiddiqui, Sahabjada
dc.contributor.authorAhmad, Ejaz [UNESP]
dc.contributor.authorGupta, Madu
dc.contributor.authorRawat, Vineeta
dc.contributor.authorShivnath, N.
dc.contributor.authorBanerjee, Monisha
dc.contributor.authorKhan, M. S.
dc.contributor.authorArshad, Mohammad
dc.contributor.institutionUniversity of Lucknow
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionIntegral University
dc.date.accessioned2015-10-21T20:58:15Z
dc.date.available2015-10-21T20:58:15Z
dc.date.issued2015-08-01
dc.description.abstractObjectives: This report highlights phytoconstituents present in Cissus quadrangularis (CQ) extract and examines biphasic (proliferative and anti-proliferative) effects of its extract on bone cell proliferation, differentiation, mineralization, ROS generation, cell cycle progression and Runx2 gene expression in primary rat osteoblasts. Materials and methods: Phytoconstituents were identified using gas chromatography-mass spectroscopy (GC-MS). Osteoblasts were exposed to different concentrations (10-100g/ml) of CQ extract and cell proliferation and cell differentiation were investigated at different periods of time. Subsequently, intracellular ROS intensity, apoptosis and matrix mineralization of osteoblasts were evaluated. We performed flow cytometry for DNA content and real-time PCR for Runx2 gene expression analysis.Results: CQ extract's approximately 40 bioactive compounds of fatty acids, hydrocarbons, vitamins and steroidal derivatives were identified. Osteoblasts exposed to varying concentrations of extract exhibited biphasic variation in cell proliferation and differentiation as a function of dose and time. Moreover, lower concentrations (10-50g/ml) of extract slightly reduced ROS intensity, although they enhanced matrix mineralization, DNA content in S phase of the cell cycle, and levels of Runx2 expression. However, higher concentrations (75-100g/ml) considerably induced the ROS intensity and nuclear condensation in osteoblasts, while it reduced mineralization level, proportion of cells in S phase and Runx2 level of the osteogenic gene.Conclusions: These findings suggest that CQ extract revealed concentration-dependent biphasic effects, which would contribute notably to future assessment of pre-clinical efficacy and safety studies.en
dc.description.affiliationUniversity of Lucknow, Molecular Endocrinology Laboratory, Department of Zoology
dc.description.affiliationUniversity of Lucknow, Molecular & Human Genetics Laboratory, Department of Zoology
dc.description.affiliationIntegral University, Department of Biosciences
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas de Araraquara
dc.description.sponsorshipUniversity Grant Commission (UGC)
dc.description.sponsorshipIndian Council of Medical Research (ICMR)
dc.description.sponsorshipCouncil of Scientific and Industrial Research (CSIR)
dc.description.sponsorshipIdUGC: 37/436-2009 SR
dc.description.sponsorshipIdCSIR: 45/26/2013/BMS/TRM
dc.description.sponsorshipIdCSIR: 09/107(0350)/2010 EMRI
dc.format.extent443-454
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1111/cpr.12195/abstract
dc.identifier.citationCell Proliferation. Hoboken: Wiley-blackwell, v. 48, n. 4, p. 443-454, 2015.
dc.identifier.doi10.1111/cpr.12195
dc.identifier.issn0960-7722
dc.identifier.urihttp://hdl.handle.net/11449/129380
dc.identifier.wosWOS:000357680600007
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofCell Proliferation
dc.relation.ispartofjcr4.936
dc.relation.ispartofsjr1,095
dc.rights.accessRightsAcesso restritopt
dc.sourceWeb of Science
dc.titleCissus quadrangularis Linn exerts dose-dependent biphasic effects: osteogenic and anti-proliferative, through modulating ROS, cell cycle and Runx2 gene expression in primary rat osteoblastsen
dc.typeArtigopt
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
dspace.entity.typePublication
relation.isDepartmentOfPublication5004bcab-94af-4939-b980-091ae9d0a19e
relation.isDepartmentOfPublication.latestForDiscovery5004bcab-94af-4939-b980-091ae9d0a19e
relation.isOrgUnitOfPublication95697b0b-8977-4af6-88d5-c29c80b5ee92
relation.isOrgUnitOfPublication.latestForDiscovery95697b0b-8977-4af6-88d5-c29c80b5ee92
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentCiências Biológicas - FCFpt

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