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Morphological alterations in salivary glands of Rhipicephalus sanguineus ticks (Acari: Ixodidae) exposed to neem seed oil with known azadirachtin concentration

dc.contributor.authorRemedio, R. N. [UNESP]
dc.contributor.authorNunes, P. H. [UNESP]
dc.contributor.authorAnholeto, L. A. [UNESP]
dc.contributor.authorOliveira, P. R. [UNESP]
dc.contributor.authorSá, I. C.G.
dc.contributor.authorCamargo-Mathias, M. I. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2018-12-11T16:40:55Z
dc.date.available2018-12-11T16:40:55Z
dc.date.issued2016-04-01
dc.description.abstractNeem (Azadirachta indica) has attracted the attention of researchers worldwide due to its repellent properties and recognized effects on the morphology and physiology of arthropods, including ticks. Therefore, this study aimed to demonstrate the effects of neem seed oil enriched with azadirachtin on salivary glands of Rhipicephalus sanguineus ticks, targets of great veterinary interest because of their ability to transmit pathogens to dogs. For this, R. sanguineus semi-engorged females were subjected to treatment with neem seed oil, with known azadirachtin concentrations (200, 400 and 600 ppm). After dissection, salivary glands were collected and evaluated through morphological techniques in light microscopy, confocal scanning laser microscopy and transmission electron microscopy, so that the possible relation between neem action and further impairment in these ectoparasites feed performance could be established. Neem oil demonstrated a clear dose-dependent effect in the analyzed samples. The agranular (type I) and granular acini (types II and III) showed, particularly in individuals treated with the highest concentrations of the product, cells with irregular shape, intense cytoplasmic disorganization and vacuolation, dilation of rough endoplasmic reticulum lumen, besides alterations in mitochondrial intermembrane space. These morphological damages may indicate modifications in salivary glands physiology, demonstrating the harmful effects of compounds present in neem oil on ticks. These results reinforce the potential of neem as an alternative method for controlling R. sanguineus ticks, instead of synthetic acaricides.en
dc.description.affiliationUNESP, Avenida 24 A, 1515
dc.description.affiliationUFSCar Rodovia Washington Luís Km 235
dc.description.affiliationUnespUNESP, Avenida 24 A, 1515
dc.format.extent19-31
dc.identifierhttp://dx.doi.org/10.1016/j.micron.2016.01.004
dc.identifier.citationMicron, v. 83, p. 19-31.
dc.identifier.doi10.1016/j.micron.2016.01.004
dc.identifier.file2-s2.0-84957057716.pdf
dc.identifier.issn0968-4328
dc.identifier.scopus2-s2.0-84957057716
dc.identifier.urihttp://hdl.handle.net/11449/168355
dc.language.isoeng
dc.relation.ispartofMicron
dc.relation.ispartofsjr0,624
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAcaricide
dc.subjectBrown dog tick
dc.subjectControl
dc.subjectMorphology
dc.subjectPhytochemical
dc.titleMorphological alterations in salivary glands of Rhipicephalus sanguineus ticks (Acari: Ixodidae) exposed to neem seed oil with known azadirachtin concentrationen
dc.typeArtigo
dspace.entity.typePublication

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