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Recovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysema

dc.contributor.authorFortunato, Gustavo
dc.contributor.authorVidal, Daniel T.A.
dc.contributor.authorKlein, Wilfried
dc.contributor.authorNeto, Alberto
dc.contributor.authorAngrizani, André
dc.contributor.authorVasconcelos, Juliana F.
dc.contributor.authorKaneto, Carla
dc.contributor.authorSouza, Bruno Solano de Freitas
dc.contributor.authorRibeiro-dos-Santos, Ricardo
dc.contributor.authorSoares, Milena B.P.
dc.contributor.authorMacambira, Simone G.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal da Bahia (UFBA)
dc.date.accessioned2014-05-27T11:29:00Z
dc.date.available2014-05-27T11:29:00Z
dc.date.issued2013-04-26
dc.description.abstractEmphysema is a chronic obstructive pulmonary disease characterized abnormal dilatation of alveolar spaces, which impairs alveolar gas exchange, compromising the physical capacity of a patient due to airflow limitations. Here we tested the effects of G-CSF administration in pulmonary tissue and exercise capacity in emphysematous mice. C57Bl/6 female mice were treated with elastase intratracheally to induce emphysema. Their exercise capacities were evaluated in a treadmill. Lung histological sections were prepared to evaluate mean linear intercept measurement. Emphysematous mice were treated with G-CSF (3 cycles of 200 μg/kg/day for 5 consecutive days, with 7-day intervals) or saline and submitted to a third evaluation 8 weeks after treatment. Values of run distance and linear intercept measurement were expressed as mean ± SD and compared applying a paired t-test. Effects of treatment on these parameters were analyzed applying a Repeated Measures ANOVA, followed by Tukey's post hoc analysis. p < 0.05 was considered statistically significant. Twenty eight days later, animals ran significantly less in a treadmill compared to normal mice (549.7 ± 181.2 m and 821.7 ± 131.3 m, respectively; p < 0.01). Treatment with G-CSF significantly increased the exercise capacity of emphysematous mice (719.6 ± 200.5 m), whereas saline treatment had no effect on distance run (595.8 ± 178.5 m). The PCR cytokines genes analysis did not detect difference between experimental groups. Morphometric analyses in the lung showed that saline-treated mice had a mean linear intercept significantly higher (p < 0.01) when compared to mice treated with G-CSF, which did not significantly differ from that of normal mice. Treatment with G-CSF promoted the recovery of exercise capacity and regeneration of alveolar structural alterations in emphysematous mice. © 2013.en
dc.identifierhttp://dx.doi.org/10.1016/j.pupt.2013.04.003
dc.identifier.citationPulmonary Pharmacology and Therapeutics.
dc.identifier.doi10.1016/j.pupt.2013.04.003
dc.identifier.issn1094-5539
dc.identifier.issn1522-9629
dc.identifier.scopus2-s2.0-84876456590
dc.identifier.urihttp://hdl.handle.net/11449/75174
dc.identifier.wosWOS:000333999800003
dc.language.isoeng
dc.relation.ispartofPulmonary Pharmacology and Therapeutics
dc.relation.ispartofjcr2.406
dc.relation.ispartofsjr0,860
dc.relation.ispartofsjr0,860
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectExercise capacity
dc.subjectG-CSF
dc.subjectMean linear intercept
dc.subjectPulmonary emphysema
dc.titleRecovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysemaen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dspace.entity.typePublication
unesp.author.orcid0000-0001-6099-6178[3]
unesp.author.orcid0000-0003-4553-0315[6]
unesp.author.orcid0000-0001-7549-2992[10]

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