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Different extenders in the cryopreservation of bovine epididymal spermatozoa

dc.contributor.authorPapa, Patrícia M. [UNESP]
dc.contributor.authorPapa, Frederico O. [UNESP]
dc.contributor.authorOliveira, Letícia A.
dc.contributor.authorGuasti, Priscilla N. [UNESP]
dc.contributor.authorCastilho, Caliê
dc.contributor.authorGiometti, Ines Cristina
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade do Oeste Paulista (UNOESTE)
dc.date.accessioned2015-12-07T15:32:02Z
dc.date.available2015-12-07T15:32:02Z
dc.date.issued2015
dc.description.abstractThe objective of this study was to evaluate the effects of two different egg yolk extenders incubated with or without Sperm Talp on the motility and plasma membrane integrity of cryopreserved bovine epididymal spermatozoa after freezing. Twenty-five testicles with epididymides from mature bulls were collected at the abattoir. Epididymal sperm recovery was performed by retrograde flushing using a skim milk-extender (Botu-Semen™). After recovery, sperm were incubated either without or with Sperm Talp and then submitted to centrifugation. For the freezing process, half of the testes were processed with Tris egg yolk extender, and half were processed with Botu-Bov™ egg yolk extender. Samples incubated in Sperm Talp exhibited better results than epididymal spermatozoa that were incubated without Sperm Talp (p<0.05). Both Botu-Bov™ and Tris could be utilised to freeze sperm from the bovine epididymides if the sperm were previously incubated with Sperm Talp. The extenders examined in this work did not differ in their effect on plasma membrane integrity after freezing.en
dc.description.affiliationDepartment of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, UNESP, Botucatu, SP, Brazil
dc.description.affiliationOeste Paulista University, UNOESTE, Presidente Prudente, SP, Brazil
dc.description.affiliationUnespDepartment of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, UNESP, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2009/50251-4
dc.format.extent58-63
dc.identifierhttp://dx.doi.org/10.1016/j.anireprosci.2015.08.004
dc.identifier.citationAnimal Reproduction Science, v. 161, p. 58-63, 2015.
dc.identifier.doi10.1016/j.anireprosci.2015.08.004
dc.identifier.issn1873-2232
dc.identifier.pubmed26315896
dc.identifier.urihttp://hdl.handle.net/11449/131149
dc.language.isoeng
dc.publisherElsevier B. V.
dc.relation.ispartofAnimal Reproduction Science
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectSperm Talpen
dc.subjectSemen extenderen
dc.subjectEpididymal spermatozoaen
dc.subjectBullen
dc.subjectCryopreservationen
dc.titleDifferent extenders in the cryopreservation of bovine epididymal spermatozoaen
dc.typeArtigo
dcterms.rightsHolderElsevier B. V.
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentReprodução Animal e Radiologia Veterinária - FMVZpt

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