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Sialidase Activity in the Cervicovaginal Fluid Is Associated With Changes in Bacterial Components of Lactobacillus-Deprived Microbiota

dc.contributor.authorFerreira, Carolina Sanitá Tafner [UNESP]
dc.contributor.authorMarconi, Camila [UNESP]
dc.contributor.authorParada, Cristina M. G. L. [UNESP]
dc.contributor.authorRavel, Jacques
dc.contributor.authorSilva, Marcia Guimaraes da [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal do Paraná (UFPR)
dc.contributor.institutionUniversity of Maryland School of Medicine
dc.date.accessioned2022-04-29T08:38:54Z
dc.date.available2022-04-29T08:38:54Z
dc.date.issued2022-01-13
dc.description.abstractIntroduction: Sialidase activity in the cervicovaginal fluid (CVF) is associated with microscopic findings of bacterial vaginosis (BV). Sequencing of bacterial 16S rRNA gene in vaginal samples has revealed that the majority of microscopic BV cases fit into vaginal community-state type IV (CST IV), which was recently named “molecular-BV.” Bacterial vaginosis-associated bacterial species, such as Gardnerella spp., may act as sources of CVF sialidases. These hydrolases lead to impairment of local immunity and enable bacterial adhesion to epithelial and biofilm formation. However, the impact of CVL sialidase on microbiota components and diversity remains unknown. Objective: To assess if CVF sialidase activity is associated with changes in bacterial components of CST IV. Methods: One hundred forty women were cross-sectionally enrolled. The presence of molecular-BV (CST IV) was assessed by V3–V4 16S rRNA sequencing (Illumina). Fluorometric assays were performed using 2-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (MUAN) for measuring sialidase activity in CVF samples. Linear discriminant analysis effect size (LEfSe) was performed to identify the differently enriched bacterial taxa in molecular-BV according to the status of CVF sialidase activity. Results: Forty-four participants (31.4%) had molecular-BV, of which 30 (68.2%) had sialidase activity at detectable levels. A total of 24 bacterial taxa were enriched in the presence of sialidase activity, while just two taxa were enriched in sialidase-negative samples. Conclusion: Sialidase activity in molecular-BV is associated with changes in bacterial components of the local microbiome. This association should be further investigated, since it may result in diminished local defenses against pathogens.en
dc.description.affiliationDepartment of Pathology Botucatu Medical School Sao Paulo State University (UNESP)
dc.description.affiliationDepartment of Basic Pathology Sector of Biological Sciences Federal University of Paraná (UFPR)
dc.description.affiliationDepartment of Nursing Botucatu Medical School Sao Paulo State University (UNESP)
dc.description.affiliationInstitute of Genomic Science University of Maryland School of Medicine
dc.description.affiliationUnespDepartment of Pathology Botucatu Medical School Sao Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Nursing Botucatu Medical School Sao Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.3389/fcimb.2021.813520
dc.identifier.citationFrontiers in Cellular and Infection Microbiology, v. 11.
dc.identifier.doi10.3389/fcimb.2021.813520
dc.identifier.issn2235-2988
dc.identifier.scopus2-s2.0-85123751223
dc.identifier.urihttp://hdl.handle.net/11449/230300
dc.language.isoeng
dc.relation.ispartofFrontiers in Cellular and Infection Microbiology
dc.sourceScopus
dc.subject16S rRNA
dc.subjectbacterial vaginosis
dc.subjectGardnerella
dc.subjectsialidases
dc.subjectvaginal microbiota
dc.titleSialidase Activity in the Cervicovaginal Fluid Is Associated With Changes in Bacterial Components of Lactobacillus-Deprived Microbiotaen
dc.typeArtigo
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina, Botucatupt
unesp.departmentEnfermagem - FMBpt
unesp.departmentPatologia - FMBpt

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