Publicação:
Random amplification of polymorphic DNA reveals clonal relationships among enteropathogenic Escherichia coli isolated from non-human primates and humans

dc.contributor.authorCarvalho, V. M.
dc.contributor.authorIrino, K.
dc.contributor.authorOnuma, D.
dc.contributor.authorde Castro, A. F. Pestana
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstituto Adolfo Lutz (IAL)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T15:29:52Z
dc.date.available2014-05-20T15:29:52Z
dc.date.issued2007-02-01
dc.description.abstractEnteropathogenic Escherichia coli ( EPEC) strains are important agents of infantile diarrhea all over the world, gaining even greater importance in developing countries. EPEC have also been isolated from various animal species, but most isolates belong to serotypes that differ from those recovered from humans. However, it has been demonstrated that several isolates from non- human primates belong to the serogroups and/ or serotypes related to those implicated in human disease. The objective of this study was to evaluate the genetic differences between thirteen strains isolated from non- human primates and the same number of strains isolated from human infections. Human isolates belonged to the same serogroup/ serotype as the monkey strains and the evaluation was done by analysis of random amplified polymorphic DNA. Dendrogram analysis showed that there was no clustering between human and monkey strains. Human and non- human isolates of the EPEC serotypes O127:H40 and O128:H2 shared 90 and 87% of their bands, respectively, indicating strong genomic similarity between the strains, leading to the speculation that they may have arisen from the same pathogenic clone. To our knowledge, this study is the first one comparing genomic similarity between human and non- human primate strains and the results provide further evidence that monkey EPEC strains correlate with human EPEC, as suggested in a previous investigation.en
dc.description.affiliationUniv Estadual Paulista, Fac Vet Med, Inst Ciências Saude, BR-01405 São Paulo, Brazil
dc.description.affiliationInst Adolfo Lutz Registro, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciências Biomed, Dept Microbiol, BR-05508 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Vet Med, Inst Ciências Saude, BR-01405 São Paulo, Brazil
dc.format.extent237-241
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2006005000043
dc.identifier.citationBrazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 40, n. 2, p. 237-241, 2007.
dc.identifier.doi10.1590/S0100-879X2006005000043
dc.identifier.fileWOS000243977400010.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2006005000043
dc.identifier.urihttp://hdl.handle.net/11449/39348
dc.identifier.wosWOS:000243977400010
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica (ABRADIC)
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.relation.ispartofjcr1.492
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectEscherichia colipt
dc.subjectenteropathogenic Escherichia colipt
dc.subjectattaching and effacing Escherichia colipt
dc.subjectattaching and effacingpt
dc.subjectmonkeypt
dc.subjectrandom amplified polymorphic DNApt
dc.titleRandom amplification of polymorphic DNA reveals clonal relationships among enteropathogenic Escherichia coli isolated from non-human primates and humansen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bjmbr/iaboutj.htm
dcterms.rightsHolderAssociação Bras Divulg Cientifica
dspace.entity.typePublication

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