Publicação: Endodontic Chelators Induce Nitric Oxide Expression by Murine-cultured Macrophages
dc.contributor.author | Pappen, Fernanda Geraldes | |
dc.contributor.author | Souza, Erick Miranda | |
dc.contributor.author | Giardino, Luciano | |
dc.contributor.author | Carlos, Iracilda Zepponi [UNESP] | |
dc.contributor.author | Leonardo, Mario Roberto | |
dc.contributor.author | Leonardo, Renato de Toledo [UNESP] | |
dc.contributor.institution | Universidade Federal de Pelotas (UFPEL) | |
dc.contributor.institution | Univ Ctr Maranhao | |
dc.contributor.institution | Univ Brescia | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2014-05-20T13:46:59Z | |
dc.date.available | 2014-05-20T13:46:59Z | |
dc.date.issued | 2009-06-01 | |
dc.description.abstract | Introduction: Endodontic chelators may extrude to apical tissues during instrumentation activating cellular events on periapical tissues. This study assessed in vitro the expression of nitric oxide (NO) concentrations by murine peritoneal macrophages after contact with MTAD (Dentsply/Tulsa, Tulsa, OK), Tetraclean (Ogna Laboratori Farmaceutici, Muggio, Italy), Smear Clear (Sybron Endo, Orange, CA), and EDTA (Biodinamica, Ibipora, PR, Brazil). Methods: Macrophage cells were obtained from Swiss mice after peritoneal lavage. Chelators were diluted in distilled water obtaining 12 concentrations, and MTT assay identified the concentrations, per group, displaying the highest cell viability (analysis of variance, p < 0.01). Selected concentrations were tested for NO expression using Griess reaction. Culture medium and lipopolysaccharide (LPS) were used as controls. Results: Analysis of variance and Tukey tests showed that all chelators displayed elevated NO concentrations compared with the negative control (p < 0.01). MTAD induced the lowest NO expression, followed by Tetraclean, EDTA, and Smear Clear. No difference was observed between MTAD and Tetraclean (p > 0.01), Tetraclean and EDTA (p > 0.01), and EDTA and Smear Clear (p > 0.01). LPS ranked similar to both EDTA and Smear Clear (p > 0.01). Conclusion: The tested endodontic chelators displayed severe proinflammatory effects on murine-cultured macrophages. Citric acid-based solutions induce lower No release than EDTA-based irrigants. (J Endod 2009;35:824-828) | en |
dc.description.affiliation | Univ Fed Pelotas, Sch Dent, Dept Semiol & Clin, BR-96015300 Pelotas, RS, Brazil | |
dc.description.affiliation | Univ Ctr Maranhao, Post Grad Dept, Sao Luis, MA, Brazil | |
dc.description.affiliation | Univ Brescia, Sch Dent, Dept Periodontol, Brescia, Italy | |
dc.description.affiliation | UNESP, Pharmaceut Sch, Dept Clin Anal, Araraquara, SP, Brazil | |
dc.description.affiliation | Univ São Paulo, Sch Dent Ribeirao Preto, Dept Pediat Dent Prevent & Community Dent, BR-14049 Ribeirao Preto, SP, Brazil | |
dc.description.affiliation | UNESP, Araraquara Dent Sch, Dept Restorat Dent, Araraquara, SP, Brazil | |
dc.description.affiliationUnesp | UNESP, Pharmaceut Sch, Dept Clin Anal, Araraquara, SP, Brazil | |
dc.description.affiliationUnesp | UNESP, Araraquara Dent Sch, Dept Restorat Dent, Araraquara, SP, Brazil | |
dc.format.extent | 824-828 | |
dc.identifier | http://dx.doi.org/10.1016/j.joen.2009.03.002 | |
dc.identifier.citation | Journal of Endodontics. New York: Elsevier B.V., v. 35, n. 6, p. 824-828, 2009. | |
dc.identifier.doi | 10.1016/j.joen.2009.03.002 | |
dc.identifier.issn | 0099-2399 | |
dc.identifier.uri | http://hdl.handle.net/11449/16673 | |
dc.identifier.wos | WOS:000267176000007 | |
dc.language.iso | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation.ispartof | Journal of Endodontics | |
dc.relation.ispartofjcr | 2.886 | |
dc.relation.ispartofsjr | 1,585 | |
dc.rights.accessRights | Acesso restrito | pt |
dc.source | Web of Science | |
dc.subject | Chelators | en |
dc.subject | cytotoxicity | en |
dc.subject | irrigation solutions | en |
dc.subject | macrophages | en |
dc.subject | nitric oxide | en |
dc.title | Endodontic Chelators Induce Nitric Oxide Expression by Murine-cultured Macrophages | en |
dc.type | Artigo | pt |
dcterms.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
dcterms.rightsHolder | Elsevier B.V. | |
dspace.entity.type | Publication | |
relation.isDepartmentOfPublication | a83d26d6-5383-42e4-bb3c-2678a6ddc144 | |
relation.isDepartmentOfPublication | 901124bf-5736-4432-b057-7c29dec84b50 | |
relation.isDepartmentOfPublication.latestForDiscovery | a83d26d6-5383-42e4-bb3c-2678a6ddc144 | |
relation.isOrgUnitOfPublication | 95697b0b-8977-4af6-88d5-c29c80b5ee92 | |
relation.isOrgUnitOfPublication | ca4c0298-cd82-48ee-a9c8-c97704bac2b0 | |
relation.isOrgUnitOfPublication.latestForDiscovery | 95697b0b-8977-4af6-88d5-c29c80b5ee92 | |
unesp.author.orcid | 0000-0003-2970-6683[3] | |
unesp.author.orcid | 0000-0003-2074-0834[2] | |
unesp.campus | Universidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquara | pt |
unesp.campus | Universidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquara | pt |
unesp.department | Análises Clínicas - FCF | pt |
unesp.department | Odontologia Restauradora - FOAR | pt |
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