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Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation

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dc.contributor.authorGoulart, A. J. [UNESP]
dc.contributor.authorCarmona, E. C. [UNESP]
dc.contributor.authorMonti, Rubens [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:56:04Z
dc.date.available2014-05-20T13:56:04Z
dc.date.issued2005-05-01
dc.description.abstractRhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. The purified enzyme obtained after molecular exclusion chromatography in Sephacryl S-200 HR showed optimum temperature as 45 degrees C and hydrolysis pHs optima as pH 6.0 and 9.0. Xylanase presented higher Vmax at pH 9.0 (0.87 mu mol/mg protein) than at pH 6.0 and minor Km at pH 6.0 (7.42 mg/mL)than at pH 9.0.en
dc.description.affiliationUniv Estadual Paulista, Fac Ciências Farmaceut, Dept Alimentos & Nutr, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13500900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências Farmaceut, Dept Alimentos & Nutr, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13500900 Rio Claro, SP, Brazil
dc.format.extent327-333
dc.identifierhttp://dx.doi.org/10.1590/S1516-89132005000300001
dc.identifier.citationBrazilian Archives of Biology and Technology. Curitiba-Paraná: Inst Tecnologia Parana, v. 48, n. 3, p. 327-333, 2005.
dc.identifier.doi10.1590/S1516-89132005000300001
dc.identifier.fileWOS000229613100001.pdf
dc.identifier.issn1516-8913
dc.identifier.lattes4110421764783871
dc.identifier.scieloS1516-89132005000300001
dc.identifier.scopus2-s2.0-27944503315
dc.identifier.urihttp://hdl.handle.net/11449/20054
dc.identifier.wosWOS:000229613100001
dc.language.isoeng
dc.publisherInst Tecnologia Parana
dc.relation.ispartofBrazilian Archives of Biology and Technology
dc.relation.ispartofjcr0.676
dc.relation.ispartofsjr0,281
dc.rights.accessRightsAcesso abertopt
dc.sourceWeb of Science
dc.subjectalkaline xylanasept
dc.subjectRhizopus stoloniferpt
dc.subjectsolid-state fermentationpt
dc.titlePartial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentationen
dc.typeArtigopt
dcterms.licensehttp://www.scielo.br/revistas/babt/iaboutj.htm
dcterms.rightsHolderInst Tecnologia Parana
dspace.entity.typePublication
relation.isOrgUnitOfPublication95697b0b-8977-4af6-88d5-c29c80b5ee92
relation.isOrgUnitOfPublication.latestForDiscovery95697b0b-8977-4af6-88d5-c29c80b5ee92
unesp.author.lattes4110421764783871
unesp.author.orcid0000-0002-0230-5735[2]
unesp.author.orcid0000-0002-7693-0672[1]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claropt
unesp.departmentAlimentos e Nutrição - FCFpt
unesp.departmentBioquímica e Microbiologia - IBpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas
Rio Claro - IB - Instituto de Biociências