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Antigens from Rhipicephalus sanguineus ticks elicit potent cell-mediated immune responses in resistant but not in susceptible animals

dc.contributor.authorFerreira, Beatriz R.
dc.contributor.authorSzabó, Matias J. P.
dc.contributor.authorCavassani, Karen A.
dc.contributor.authorBechara, Gervásio H.
dc.contributor.authorSilva, João S.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:20:41Z
dc.date.available2014-05-27T11:20:41Z
dc.date.issued2003-07-10
dc.description.abstractIn the present study we compared the immunological reactions between Rhipicephalus sanguineus tick-infested susceptible (dogs and mice) and tick-resistant hosts (guinea pigs), elucidating some of the components of efficient protective responses against ticks. We found that T-cells from guinea pigs infested with adult ticks proliferate vigorously in the presence of concanavalin A (ConA), whereas ConA-induced cell proliferation of tick-infested mice and dogs was significantly decreased at 43.1 and 94.0%, respectively, compared to non-infested controls. Moreover, cells from mice and dogs submitted to one or three successive infestations did not exhibit a T-cell proliferative response to tick antigens, whilst cells from thrice tick-infested guinea pigs, when cultured with either a tick extract or tick saliva, displayed a significant increase in cell proliferation. Also, we evaluated the response of tick-infested mice to a cutaneous hypersensitivity test induced by a tick extract. Tick-infested mice developed a significant immediate reaction, whereby a 29.9% increase in the footpad thickness was observed. No delayed-type hypersensitivity (DTH) reaction was detected. Finally, the differential cell count at the tick attachment site in repeatedly infested mice exhibited a 6.6- and 4.1-fold increase in the percentage of eosinophils and neutrophils, respectively, compared to non-infested animals, while a decrease of 77.0-40.9 in the percentage of mononuclear cells was observed. The results of the cutaneous hypersensitivity test and the cellular counts at the tick feeding site for mice support the view that tick-infested mice develop an immune response to R. sanguineus ticks very similar to dogs, the natural host of this species of tick, but very different from guinea pigs (resistant host), which develop a DTH reaction in addition to a basophil and mononuclear cell infiltration at the tick-attachment site. In conclusion, saliva introduced during tick infestations reduces the ability of a susceptible animal host to respond to tick antigens that could stimulate a protective immune response. As a consequence, the animals present a lack of DTH response and disturbed cellular migration to tick feeding site, which can represent a deficient response against ticks. © 2003 Elsevier B.V. All rights reserved.en
dc.description.affiliationDept. of Biochemistry and Immunology Sch. of Med. of Ribeirão Preto University of São Paulo, 14.049-900 Ribeirão Preto, SP
dc.description.affiliationDepartment of Veterinary Pathology Sch. of Vet. Science of Jaboticabal Univ. of the Stt. of São Paulo, 14.870-000 Jaboticabal, SP
dc.format.extent35-48
dc.identifierhttp://dx.doi.org/10.1016/S0304-4017(03)00190-0
dc.identifier.citationVeterinary Parasitology, v. 115, n. 1, p. 35-48, 2003.
dc.identifier.doi10.1016/S0304-4017(03)00190-0
dc.identifier.issn0304-4017
dc.identifier.scopus2-s2.0-0037967850
dc.identifier.urihttp://hdl.handle.net/11449/67358
dc.language.isoeng
dc.relation.ispartofVeterinary Parasitology
dc.relation.ispartofjcr2.422
dc.relation.ispartofsjr1,275
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCellular immunity
dc.subjectDog
dc.subjectHypersensitivity
dc.subjectImmune response
dc.subjectMice model
dc.subjectRhipicephalus sanguineus
dc.subjectanimal experiment
dc.subjectcell count
dc.subjectcell infiltration
dc.subjectcell proliferation
dc.subjectcellular immunity
dc.subjectcontrolled study
dc.subjectdog
dc.subjecteosinophil
dc.subjectfemale
dc.subjectguinea pig
dc.subjectinfection resistance
dc.subjectinfection sensitivity
dc.subjectmononuclear cell
dc.subjectmouse
dc.subjectneutrophil
dc.subjectnonhuman
dc.subjectsaliva
dc.subjectskin allergy
dc.subjectskin test
dc.subjectT lymphocyte
dc.subjecttick
dc.subjectAnimals
dc.subjectAntigens
dc.subjectCell Division
dc.subjectConcanavalin A
dc.subjectDisease Susceptibility
dc.subjectDog Diseases
dc.subjectDogs
dc.subjectFemale
dc.subjectGuinea Pigs
dc.subjectHost-Parasite Relations
dc.subjectIxodidae
dc.subjectMale
dc.subjectMice
dc.subjectMice, Inbred C3H
dc.subjectSaliva
dc.subjectSkin
dc.subjectT-Lymphocytes
dc.subjectTick Infestations
dc.subjectAcari
dc.subjectAnimalia
dc.subjectCanis familiaris
dc.subjectCavia
dc.subjectRhipicephalus
dc.titleAntigens from Rhipicephalus sanguineus ticks elicit potent cell-mediated immune responses in resistant but not in susceptible animalsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentPatologia Veterinária - FCAVpt

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