Publicação: Microplate alamarblue assay for paracoccidioides susceptibility testing
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CLSI method M27-A3 is not available for use with dimorphic fungi, such as those of the Paracoccidioides genus. In this study, we developed a microdilution method and added the alamarBlue reagent to test the responses of Paracoccidioides brasiliensis and Paracoccidioides lutzii against amphotericin B and itraconazole antifungals. The test proved to be sensitive, practical, and inexpensive and can be used to monitor the activity of low-growth microorganisms and their response to various drugs. © 2013, American Society for Microbiology.
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alamarBlue, amphotericin B, itraconazole, reagent, unclassified drug, antifungal activity, antifungal susceptibility, broth dilution, controlled study, fungal strain, human, intermethod comparison, minimum inhibitory concentration, nonhuman, Paracoccidioides, Paracoccidioides brasiliensis, Paracoccidioides lutzii, priority journal, Amphotericin B, Antifungal Agents, Humans, Itraconazole, Microbial Sensitivity Tests, Oxazines, Staining and Labeling, Xanthenes
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Inglês
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Journal of Clinical Microbiology, v. 51, n. 4, p. 1250-1252, 2013.
