Microplate alamarblue assay for paracoccidioides susceptibility testing
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American Society for Microbiology
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Resumo
CLSI method M27-A3 is not available for use with dimorphic fungi, such as those of the Paracoccidioides genus. In this study, we developed a microdilution method and added the alamarBlue reagent to test the responses of Paracoccidioides brasiliensis and Paracoccidioides lutzii against amphotericin B and itraconazole antifungals. The test proved to be sensitive, practical, and inexpensive and can be used to monitor the activity of low-growth microorganisms and their response to various drugs. © 2013, American Society for Microbiology.
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alamarBlue , amphotericin B , itraconazole , reagent , unclassified drug , antifungal activity , antifungal susceptibility , broth dilution , controlled study , fungal strain , human , intermethod comparison , minimum inhibitory concentration , nonhuman , Paracoccidioides , Paracoccidioides brasiliensis , Paracoccidioides lutzii , priority journal , Amphotericin B , Antifungal Agents , Humans , Itraconazole , Microbial Sensitivity Tests , Oxazines , Staining and Labeling , Xanthenes
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Journal of Clinical Microbiology, v. 51, n. 4, p. 1250-1252, 2013.
