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Production of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industry

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dc.contributor.authorFreitas, Telma Luisa de [UNESP]
dc.contributor.authorMonti, Rubens [UNESP]
dc.contributor.authorContiero, Jonas [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:21:12Z
dc.date.available2014-05-27T11:21:12Z
dc.date.issued2004-12-01
dc.description.abstractGCTase production by a new strain of Bacillus alkalophillc CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na 2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55°C for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg + and Cu+ showed a strong enzyme inhibition.en
dc.description.affiliationDepartamento de Bioquímica e Tecnologia Universidade Estadual Paulista Júlio Mesquita Filho, Araraquara, SP
dc.description.affiliationDepartamento de Alimentos e Nutrição Faculdade De/FCF Universidade Estadual Paulista Júlio Mesquita Filho, Araraquara, SP
dc.description.affiliationDepartamento de Bioquímica e Microbiologia Instituto de Biociências Universidade Estadual Paulista Júlio Mesquita Filho, Rio Claro, SP
dc.description.affiliationInstituto de Biociências de Rio Claro UNESP, Caixa Postal 199, 13506-900, Rio Claro, SP
dc.description.affiliationUnespDepartamento de Bioquímica e Tecnologia Universidade Estadual Paulista Júlio Mesquita Filho, Araraquara, SP
dc.description.affiliationUnespDepartamento de Alimentos e Nutrição Faculdade De/FCF Universidade Estadual Paulista Júlio Mesquita Filho, Araraquara, SP
dc.description.affiliationUnespDepartamento de Bioquímica e Microbiologia Instituto de Biociências Universidade Estadual Paulista Júlio Mesquita Filho, Rio Claro, SP
dc.description.affiliationUnespInstituto de Biociências de Rio Claro UNESP, Caixa Postal 199, 13506-900, Rio Claro, SP
dc.format.extent255-260
dc.identifierhttp://dx.doi.org/10.1590/S1517-83822004000200015
dc.identifier.citationBrazilian Journal of Microbiology, v. 35, n. 3, p. 255-260, 2004.
dc.identifier.doi10.1590/S1517-83822004000200015
dc.identifier.file2-s2.0-19944363225.pdf
dc.identifier.issn1517-8382
dc.identifier.lattes9859154979447005
dc.identifier.scieloS1517-83822004000200015
dc.identifier.scopus2-s2.0-19944363225
dc.identifier.urihttp://hdl.handle.net/11449/67995
dc.identifier.wosWOS:000234802600015
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Microbiology
dc.relation.ispartofjcr1.810
dc.relation.ispartofsjr0,630
dc.rights.accessRightsAcesso abertopt
dc.sourceScopus
dc.subjectβ-cyclodextrin
dc.subjectBacillus alkalophilic CGII
dc.subjectCGTase
dc.subjectEnzyme activity
dc.subjectStarch hydrolysis
dc.subjectManihot esculenta
dc.titleProduction of CGTase by a bacillus Alkalophilic CGII strain isolated from wastewater of a manioc flour industryen
dc.typeArtigopt
dcterms.licensehttp://www.scielo.br/revistas/bjm/paboutj.htm
dspace.entity.typePublication
relation.isOrgUnitOfPublication95697b0b-8977-4af6-88d5-c29c80b5ee92
relation.isOrgUnitOfPublication.latestForDiscovery95697b0b-8977-4af6-88d5-c29c80b5ee92
unesp.author.lattes9859154979447005
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Química, Araraquarapt
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claropt
unesp.departmentAlimentos e Nutrição - FCFpt
unesp.departmentBioquímica e Microbiologia - IBpt
unesp.departmentBioquímica e Tecnologia - IQpt

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Araraquara - IQAR - Instituto de Química
Araraquara - FCF - Faculdade de Ciências Farmacêuticas
Rio Claro - IB - Instituto de Biociências