Effects of Hydrolysed Poultry Byproduct Meal on Metabolic, Inflammatory and Oxidative Parameters in Cats

Abstract

Hydrolysed proteins are of interest owing to their potential effects on metabolic and physiological responses, low allergenicity and high digestibility. This study aimed to evaluate the use of hydrolysed poultry byproduct meal (HPM) as a replacement for conventional poultry byproduct meal (PBM) as a protein source and to study its effects on serum cytokines, angiotensin-converting enzyme (ACE) activity, serum antioxidant parameters, blood pressure, and urinary parameters in cats. The replacement of PBM with HPM was evaluated using five formulations with similar chemical compositions: control (PBM as the sole protein source) and the inclusion of 5%, 10%, 20%, and 30% HPM (on an as-fed basis). Thirty cats were distributed into two randomised blocks of 15 cats, with 3 cats per diet in each block, totalling 6 cats per food. After 10 days of diet adaptation, the urine of the cats was quantitatively collected from Days 11 to 15, and on the day 21 blood samples were collected and blood pressure was evaluated. Data were subjected to analysis of variance and the means were compared by polynomial contrasts. Non-parametrically distributed variables were compared using the Kruskal–Wallis test (p < 0.05). All the cats remained healthy throughout the study period. Diet did not affect urine volume; intake and excretion of sodium, potassium, and chloride; blood pressure; ACE plasma activity; or serum triglyceride and cholesterol levels (p > 0.05). Among the oxidative parameters, there was a quadratic increase in lipid peroxidation and glutathione S-transferase (higher values at 10% HPM) (p < 0.05). A quadratic increase was observed for steam cell factor, interferon-γ, IL-4, and IL-8 (p < 0.05), with higher values for cats fed 5% and 10% HPM diets. A nonparametric distribution with higher values for cats fed the 5% HPM diet was observed for monocyte chemoattractant protein-1; tumour necrosis factor-α; IL-2; and regulated upon activation, normal T cell expressed and secreted (RANTES) (p < 0.05). In conclusion, we observed that the intake of diets containing 5% and 10% HPM stimulated the antioxidant system and the secretion of several cytokines involved in the preparation and function of the immune system, suggesting potential biological functions with implications for cat health deserving further investigation.