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Isolation of natural inhibitors of papain obtained from Carica papaya latex

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dc.contributor.authorMonti, Rubens [UNESP]
dc.contributor.authorContiero, Jonas [UNESP]
dc.contributor.authorGoulart, Antonio José [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstituto de Biociências
dc.date.accessioned2014-05-27T11:21:12Z
dc.date.available2014-05-27T11:21:12Z
dc.date.issued2004-12-01
dc.description.abstractStudies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kinetics data showed non-competitive inhibition (inhibitor I) and uncompetitive (inhibitors II and P -III).en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Farmacêuticas Departamento de Alimentos e Nutrição, Rod. Araraquara-Jaú, Km 1, CEP 14801-902, Araraquara - SP
dc.description.affiliationInstituto de Biociências Departamento de Bioquímica e Microbiologia, Rua 24-A, 1515, CEP 13500-900, Rio Claro - SP
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Farmacêuticas Departamento de Alimentos e Nutrição, Rod. Araraquara-Jaú, Km 1, CEP 14801-902, Araraquara - SP
dc.format.extent747-754
dc.identifierhttp://dx.doi.org/10.1590/S1516-89132004000500010
dc.identifier.citationBrazilian Archives of Biology and Technology, v. 47, n. 5, p. 747-754, 2004.
dc.identifier.doi10.1590/S1516-89132004000500010
dc.identifier.file2-s2.0-19944371762.pdf
dc.identifier.issn1516-8913
dc.identifier.lattes5962867835836749
dc.identifier.scieloS1516-89132004000500010
dc.identifier.scopus2-s2.0-19944371762
dc.identifier.urihttp://hdl.handle.net/11449/67993
dc.identifier.wosWOS:000225349100010
dc.language.isoeng
dc.relation.ispartofBrazilian Archives of Biology and Technology
dc.relation.ispartofjcr0.676
dc.relation.ispartofsjr0,281
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectEsterase activity
dc.subjectNatural papain inhibitor
dc.subjectProtease inhibitor
dc.titleIsolation of natural inhibitors of papain obtained from Carica papaya latexen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/babt/paboutj.htm
dspace.entity.typePublication
unesp.author.lattes5962867835836749
unesp.author.orcid0000-0002-7693-0672[3]
unesp.author.orcid0000-0003-1215-6400[2]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentAlimentos e Nutrição - FCFpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas