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Genotoxic effect of photodynamic therapy mediated by curcumin on Candida albicans

dc.contributor.authorCarmello, Juliana Cabrini [UNESP]
dc.contributor.authorPavarina, Ana Claudia [UNESP]
dc.contributor.authorOliveira, Rui
dc.contributor.authorJohansson, Bjorn
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-08-06T16:13:05Z
dc.date.available2015-08-06T16:13:05Z
dc.date.issued2015
dc.description.abstractPhotodynamic therapy (PDT) is a promising method for localized and specific inactivation of fungi and bacteria. A nontoxic light-sensitive compound is taken up by cells, which are then exposed selectively to light, which activates toxicity of the compound. We investigated the potential of sublethal PDT using light-sensitive curcumin (CUR) in combination with blue (455 nm) light to promote reactive oxygen species (ROS) formation in the form of singlet oxygen and DNA damage of Candida albicans. Surprisingly, CUR-mediated PDT but also light alone caused significantly longer comet tails, an indication of DNA damage of C. albicans when compared with the negative control. The intracellular ROS production was also significantly higher for the group treated only with light. However, PDT compared to blue light alone significantly slowed DNA repair. Comet tails decreased during 30 min visualized as a 90% reduction in length in the absence of light for cells treated with light alone, while comet tails of cells treated with PDT only diminished in size about 45%. These results indicate that complex mechanisms may result in PDT in a way that should be considered when choosing the photosensitive compound and other aspects of the treatment design.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Materiais Odontológicos e Prótese, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801903, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Materiais Odontológicos e Prótese, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801903, SP, Brasil
dc.identifierhttp://femsyr.oxfordjournals.org/content/15/4/fov018.long
dc.identifier.citationFEMS Yeast Research, v. 15, n. 4, 2015.
dc.identifier.doi10.1093/femsyr/fov018
dc.identifier.issn1567-1364
dc.identifier.lattes8867670539105403
dc.identifier.urihttp://hdl.handle.net/11449/125775
dc.language.isoeng
dc.relation.ispartofFEMS Yeast Research
dc.relation.ispartofsjr1,308
dc.rights.accessRightsAcesso restritopt
dc.sourceCurrículo Lattes
dc.subjectPhotoinactivationen
dc.subjectFungien
dc.subjectPhotosensitizer agenten
dc.subjectGenotoxicityen
dc.titleGenotoxic effect of photodynamic therapy mediated by curcumin on Candida albicansen
dc.typeArtigopt
dspace.entity.typePublication
relation.isDepartmentOfPublication3936e2e2-946a-42ab-8b9d-9521513200fc
relation.isDepartmentOfPublication.latestForDiscovery3936e2e2-946a-42ab-8b9d-9521513200fc
relation.isOrgUnitOfPublicationca4c0298-cd82-48ee-a9c8-c97704bac2b0
relation.isOrgUnitOfPublication.latestForDiscoveryca4c0298-cd82-48ee-a9c8-c97704bac2b0
unesp.author.lattes8867670539105403[2]
unesp.author.orcid0000-0002-9231-1994[2]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
unesp.departmentMateriais Odontológicos e Prótese - FOARpt

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