Logotipo do repositório
 

Publicação:
Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays

Página do item simplificado
dc.contributor.authorCruz, Vanessa P. [UNESP]
dc.contributor.authorVera, Manuel
dc.contributor.authorPardo, Belén G.
dc.contributor.authorTaggart, John
dc.contributor.authorMartinez, Paulino
dc.contributor.authorOliveira, Claudio [UNESP]
dc.contributor.authorForesti, Fausto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Girona
dc.contributor.institutionUniversidad de Santiago de Compostela
dc.contributor.institutionUniversity of Stirling
dc.date.accessioned2018-12-11T17:04:34Z
dc.date.available2018-12-11T17:04:34Z
dc.date.issued2017-05-01
dc.description.abstractSingle nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226 774 reads (65.5 Mb) were obtained (mean read length 289 ± 183 bp) detecting a total of 5399 contigs (mean contig length: 396 ± 91 bp). Mining this data set, a panel of 143 in silico SNPs was selected. Eighty-two of these SNPs were successfully validated and 61 were polymorphic: 14 in P. falkneri, 21 in P. motoro, 3 in both species and 26 fixed for alternative variants in both species, thus being useful for population analyses and hybrid detection.en
dc.description.affiliationLaboratório de Biologia e Genética de Peixes Instituto de Biociências de Botucatu UNESP
dc.description.affiliationLaboratori d'Ictiologia Genètica Department of Biology Faculty of Sciences University of Girona, Campus de Montilivi
dc.description.affiliationDepartamento de Genética Facultad de Veterinaria Universidad de Santiago de Compostela
dc.description.affiliationInstitute of Aquaculture University of Stirling, Pathfoot Building
dc.description.affiliationUnespLaboratório de Biologia e Genética de Peixes Instituto de Biociências de Botucatu UNESP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundación Banco Santander
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent550-556
dc.identifierhttp://dx.doi.org/10.1111/1755-0998.12564
dc.identifier.citationMolecular Ecology Resources, v. 17, n. 3, p. 550-556, 2017.
dc.identifier.doi10.1111/1755-0998.12564
dc.identifier.issn1755-0998
dc.identifier.issn1755-098X
dc.identifier.scopus2-s2.0-84979554019
dc.identifier.urihttp://hdl.handle.net/11449/173299
dc.language.isoeng
dc.relation.ispartofMolecular Ecology Resources
dc.relation.ispartofsjr3,355
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectconservation tools
dc.subjectgenetic fish
dc.subjecthybridization
dc.subjectNGS techniques
dc.subjectPotamotrygon
dc.subjectSNPs
dc.titleIdentification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingraysen
dc.typeArtigo
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentGenética - IBBpt

Arquivos

Coleções

Botucatu - IBB - Instituto de Biociências