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Three-dimensional lung parenchyma model for studies of Aspergillus fumigatus infection and antifungal treatment

dc.contributor.authorFortes, Bruna Nakanishi
dc.contributor.authorWirth, Fernanda
dc.contributor.authordos Santos, Aline Martins [UNESP]
dc.contributor.authorChorilli, Marlus [UNESP]
dc.contributor.authorFreitas, Vanessa Morais
dc.contributor.authorFarias, Jennifer
dc.contributor.authorChambergo, Felipe S
dc.contributor.authorNunes C Dantas, Viviane Abreu
dc.contributor.authorIshida, Kelly
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2025-04-29T19:30:33Z
dc.date.issued2024-01-01
dc.description.abstractAim: This work aims to standardize the three-dimensional hydroxyethyl-alginate-gelatin (HAG) scaffold as a model to evaluate Aspergillus fumigatus biofilm and antifungal treatments. Methods: The scaffold was characterized by physical, rheological and microscopic analyses; the antibiofilm action was evaluated by determination of cfu and metabolic activity. Results: The scaffold was non-toxic showing stability in aqueous media, swelling capacity, elasticity and had homogeneously distributed pores averaging 190 μm. The A. fumigatus biofilm established itself very well on the scaffold and treatment with amphotericin B and voriconazole reduced viable cells and metabolic activity. Conclusion: The HAG scaffold proved to be a model to mimic lung parenchyma, suitable for establishing a 3D biofilm culture of A. fumigatus and evaluating the efficacy of antifungals.en
dc.description.affiliationInstitute of Biomedical Sciences University of São Paulo
dc.description.affiliationSchool of Pharmaceutical Sciences São Paulo State University–Jaú Highway
dc.description.affiliationSchool of Arts Sciences & Humanities University of São Paulo
dc.description.affiliationUnespSchool of Pharmaceutical Sciences São Paulo State University–Jaú Highway
dc.format.extent1203-1216
dc.identifierhttp://dx.doi.org/10.1080/17460913.2024.2371926
dc.identifier.citationFuture Microbiology, v. 19, n. 14, p. 1203-1216, 2024.
dc.identifier.doi10.1080/17460913.2024.2371926
dc.identifier.issn1746-0921
dc.identifier.issn1746-0913
dc.identifier.scopus2-s2.0-85198531595
dc.identifier.urihttps://hdl.handle.net/11449/303741
dc.language.isoeng
dc.relation.ispartofFuture Microbiology
dc.sourceScopus
dc.subjectamphotericin B
dc.subjectantifungals
dc.subjectAspergillus fumigatus
dc.subjectbiofilm
dc.subjectlung epithelial cell
dc.subjectscaffold
dc.subjectthree-dimensional cell culture
dc.subjectvoriconazole
dc.titleThree-dimensional lung parenchyma model for studies of Aspergillus fumigatus infection and antifungal treatmenten
dc.typeArtigopt
dspace.entity.typePublication
relation.isOrgUnitOfPublication95697b0b-8977-4af6-88d5-c29c80b5ee92
relation.isOrgUnitOfPublication.latestForDiscovery95697b0b-8977-4af6-88d5-c29c80b5ee92
unesp.author.orcid0000-0002-4602-0926[9]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt

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