Publicação: Short communication: Follicle superstimulation before ovum pick-up for in vitro embryo production in Holstein cows
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Elsevier B.V.
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The objective was to evaluate in vitro embryo production (IVEP) in nonlactating Holstein cows after ovarian superstimulation. Cows were randomly assigned in a crossover design to 1 of 2 groups: control (n = 35), which was not synchronized and not treated with hormones before ovum pick-up (OPU), or hormone-treated (n = 35), in which wave emergence was synchronized and animals treated with porcine (p)-FSH in the presence of norgestomet before OPU. In the hormone-treated group, all follicles = 7 mm in diameter were aspirated for synchronization of wave emergence and cows received a norgestomet ear implant. After 36 h, treatment with p-FSH (6 doses of 40 mg each, 12 h apart, i.m.) started. Ovum pick-up from follicles >2 mm in diameter was performed 44 h after the last pFSH (coasting). Then, IVEP was performed. The total number of cumulus-oocyte complexes recovered (16.0 vs. 20.5 +/- 2.2) and number of grades I to III (viable) oocytes (10.7 vs. 12.3 +/- 1.6) did not differ between hormone-treated and control groups Additionally, no differences were found in the number of blastocysts per cow per OPU (3.0 vs. 2.6 +/- 0.5) or in blastocyst rates (17.1 vs. 12.2 +/- 2.4%) between hormone-treated and control, respectively. Thus, in this study, ovarian follicle superstimulation with p-FSH followed by coasting in nonlactating Holstein cows that had synchronization of wave emergence and progestin supplementation did not improve oocyte quality or IVEP compared to no hormonal treatment.
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biotechnology, blastocyst, cattle, oocyte
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Inglês
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Journal Of Dairy Science. New York: Elsevier Science Inc, v. 99, n. 11, p. 9307-9312, 2016.
