Publicação: Viability of ovine spermatozoa collected from epididymides stored at 18 degrees-25 degrees C for 48 hours post mortem
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Arquivo Brasileiro Medicina Veterinaria Zootecnia
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The objectives of this study were to verify the time during which viable ovine spermatozoa could be recovered from the cauda epididymis kept at ambient temperature (18-25 degrees C). Sperm collected in an artificial vagina (AV) were used as control. Spermatozoa samples were collected with an AV and from epididymis at 0 (G0), 6 (G6), 12 (G12), 24 (G24), and 48 (G48) hours post mortem. Total motility (TM), progressive motility (PM), hypo-osmotic membrane integrity test (HOST) and morphological changes were assessed. TM decreased (P<0.05) from 24 hours post mortem (70.0 +/- 1.9%) compared to AV (86.4 +/- 1.0%). PM decreased (P<0.05) from 12 hours after death (31.3 +/- 4.0%) compared to AV group (73.2 +/- 1.4%). The percentage of viable cells in HOST decreased (P<0.05) in the G48 (60.0 +/- 8.9%). Spermatozoa recovery was lower (P<0.05) 48 hours after death (2064.2 +/- 230.7 x 10(6) spermatozoa) compared to G0(2623.6 +/- 288.4 x 10(6) spermatozoa). In conclusion, under the conditions of this study, it would be possible to use epididymal spermatozoa recovered up to 24 hours after death for artificial insemination or in vitro fertilization; however, fertility trials are necessary to prove this hypothesis.
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ram, epididymis, room temperature
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Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 70, n. 4, p. 1023-1028, 2018.
