Avaliação de mediadores da resposta inflamatória na interface materno-fetal: Estudo in vitro de infecção bacteriana, isolada ou em combinação com Mycoplasma hominis, em células amnióticas epiteliais imortalizadas

Abstract

To evaluate the mediators of the inflammatory response, in an in vitro model, of immortalized epithelial amniotic cells (hFM-AECs) challenged by different bacterial species, alone and in combinations with Mycoplasma hominis, and the interference of this stimulus on the epithelial-mesenchymal transition. In vitro assay with hFM-AECs challenged with different species were performed in diferente moments. Supernatants were collected for quantification of IL-1β, IL-6, IL-8, IL-10, IL-13, GM-CSF, TNF-α, HBD-1, HBD-2 and HBD-3 by ELISA. For analyses of EMT, the cell shape index was applied. The data were subjected to a mixed effects model combined with parametric bootstrapping.One Way ANOVA and paired T-test were used for the EMT analysis. (a) Elevated levels of IL-6, IL-8, GM-CSF and HBD-1 were detected in relation to the reference when stimulated with Fusobacterium nucleatum, alone or in combination with M. hominis; (b) IL-13 levels were decreased in relation to the reference after stimulation with F. nucleatum, Bacteroides fragilis, Peptostreptococcus anaerobius and Streptococcus agalactiae alone and in combination with M. hominis; (c) Different isolates or combination with M. hominis stimulated EMT. F. nucleatum alone or in combination with M. hominis, have an important capacity to induce a characteristic inflammatory signature. Bacterial combinations stimulate EMT.