Publication: Avaliação da reação em cadeia pela polimerase para diagnóstico da leishmaniose visceral em sangue de cães
Loading...
Date
Advisor
Coadvisor
Graduate program
Undergraduate course
Journal Title
Journal ISSN
Volume Title
Publisher
Brazilian Coll Veterinary Parasitology
Type
Article
Access right
Acesso aberto
Abstract
Canine and human visceral leishmaniasis is endemic in several States of Brazil, and it is associated with infected dogs and the presence of the vector. Aiming at using polymerase chain reaction as a diagnostic tool in dogs, we amplified a 120bp fragment from kDNA of Leishmania spp. by PCR in blood samples. The lower detection limit observed was 0.1 parasites per 500 mu L of blood, which is a highly satisfactory result. on the other hand, PCR evaluation in 166 blood samples of dogs from Poxoreo, MS, Brazil, resulted in 55% sensitivity and 66.3% specificity, considering indirect imunnofluorescent test as gold standard.
Description
Keywords
canine visceral leishmaniasis, diagnosis, deoxyribomicleic acid (DNA), polymerase chain reaction (PCR), dog
Language
Portuguese
Citation
Revista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 16, n. 1, p. 5-9, 2007.
