Publicação: Bioethanol strains of Saccharomyces cereuisiae characterised by microsatellite and stress resistance
Carregando...
Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Soc Brasileira Microbiologia
Tipo
Artigo
Direito de acesso
Acesso aberto
Resumo
Strains of Saccharomyces cereoisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cereoisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cereu isiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The results obtained from the phenotypic tests by Principal-Component Analysis revealed that unlike the smooth colonies, the rough colonies of S. cereu isiae exhibit an enhanced resistance to stressful conditions resulting from the presence of excessive glucose and ethanol and high sedimentation rate. The microsatellite analysis was not successful to distinguish between the colony phenotypes as phenotypic assays. The relevant industrial strain PE-2 was observed in close genetic proximity to rough-colony although it does not display this colony morphology. A unique genetic pattern specific to a particular phenotype remains elusive. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
Descrição
Palavras-chave
Fermentation, Stresses, Yeasts, Microsatellite, Contaminants
Idioma
Inglês
Como citar
Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 48, n. 2, p. 268-274, 2017.
