Proinflammatory cytokine production from NOK-SI keratinocytes after exposure to denture adhesives

Abstract

Statement of problem. Although the cytotoxicity of denture adhesives has been widely assessed, data on the release of proinflammatory cytokines from oral epithelial cells are still scarce. Purpose. The purpose of this in vitro study was to evaluate the proinflammatory cytokines interleukin (IL) 10, IL-6, and tumor necrosis factor alpha (TNF-alpha) released from human oral keratinocytes after exposure to denture adhesives. Material and methods. Eluates of the Ultra Corega cream, Corega powder Fixador Ultra, and Corega strip denture adhesives were created at 1% wt/vol after 24 hours of exposure in a Dulbecco modified Eagle medium culture. The release of IL-1 beta, IL-6, and TNF-alpha was measured using a sandwich enzyme-linked immunosorbent assay after the oral epithelial cells (NOK-SI) had been exposed to the eluates for 3, 6, and 24 hours (n=6). Results. IL-1 beta release from the strip was higher at 3 hours compared with the control (Bonferroni post hoc test, P=.016). IL-6 releases from the cream and powder denture adhesives at 6 and 24 hours were higher than for the controls (Bonferroni post hoc test, P<.05). TNF-alpha release was not detectable in some groups and periods. Conclusions. In long periods, denture adhesive strips do not induce any change on cytokine release from human oral keratinocytes.