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Immunization of Wistar female rats with 255-Gy-irradiated Toxoplasma gondii: Tissue parasitic load and lactogenic quantification

dc.contributor.authorCamossi, Lucilene Granuzzio [UNESP]
dc.contributor.authorFornazari, Felipe [UNESP]
dc.contributor.authorRichini-Pereira, Virginia Bodelao [UNESP]
dc.contributor.authorSilva, Rodrigo Costa da [UNESP]
dc.contributor.authorFerreira Cardia, Daniel Fontana [UNESP]
dc.contributor.authorLangoni, Helio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-10-21T13:09:50Z
dc.date.available2015-10-21T13:09:50Z
dc.date.issued2015-07-01
dc.description.abstractToxoplasma gondii is one of the most significant parasite, due its importance in veterinary medicine and in public health, considered a food-borne pathogens, there is no available drug treatments to eliminate it from animal tissue, this reinforce the search for a vaccine against this parasite. This study was aimed to evaluate the dynamic of the distribution of T gondii in tissues of female Wistar rats and their milk, after the immunization by oral rote with irradiated tachyzoites. One week after pregnancy confirmation, rats was challenged by gavage with T. gondii bradyzoites, oocysts or tachyzoites of T. gondii. Forty-eight pregnant rats were grouped as follows: immunized and challenged with bradyzoites (BZ*); non-immunized and challenged with bradyzoites (BZ); immunized and challenged with oocysts (OC*); non-immunized and challenged with oocysts (OC); immunized and challenged with tachyzoites (TZ*); non-immunized and challenged with tachyzoites (17); only immunized (I); control group (C). After parturition, milk samples were collected for 3 weeks and then rats were sacrificed and the tissues and milk samples were researched for T gondii parasite load determined by the quantitative PCR (qPCR). It was verified that the immunization with irradiated tachyzoites of T. gondii induced the reduction of parasitic load in muscle samples in rats challenged by bradyzoites and oocysts, although not enabled the development of sterile immunity. The detection of parasite DNA in milk was found throughout the lactation period, from immunized and non-immunized rats, however no differences were found in the parasite load caused by immunization. (C) 2015 Published by Elsevier Inc.en
dc.description.affiliationDepartamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, UNESP Univ Estadual Paulista, Campus de Botucatu, Botucatu, SP, Brazil
dc.description.affiliationUnespDepartamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, UNESP Univ Estadual Paulista, Campus de Botucatu, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2010/02141-2
dc.format.extent163-169
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0014489415001216
dc.identifier.citationExperimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 154, p. 163-169, 2015.
dc.identifier.doi10.1016/j.exppara.2015.04.023
dc.identifier.issn0014-4894
dc.identifier.lattes5326072118518067
dc.identifier.urihttp://hdl.handle.net/11449/128435
dc.identifier.wosWOS:000355640500024
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofExperimental Parasitology
dc.relation.ispartofjcr1.821
dc.relation.ispartofsjr0,635
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectToxoplasmosisen
dc.subjectImmunizationen
dc.subjectWistar ratsen
dc.subjectParasite burdenen
dc.subjectMilken
dc.titleImmunization of Wistar female rats with 255-Gy-irradiated Toxoplasma gondii: Tissue parasitic load and lactogenic quantificationen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
unesp.author.lattes5326072118518067
unesp.author.orcid0000-0001-9319-7516[4]
unesp.author.orcid0000-0001-8312-7397[3]
unesp.author.orcid0000-0001-5127-0762[6]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentHigiene Veterinária e Saúde Pública - FMVZpt

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