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Immunomodulation and protection induced by DNA-hsp65 vaccination in an animal model of arthritis

dc.contributor.authorSantos-Junior, R. R.
dc.contributor.authorSartori, A.
dc.contributor.authorDe Franco, M.
dc.contributor.authorFilho, OGR
dc.contributor.authorCoelho-Castelo, AAM
dc.contributor.authorBonato, VLD
dc.contributor.authorCabrera, WHK
dc.contributor.authorIbanez, O. M.
dc.contributor.authorSilva, C. L.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstituto Butantan
dc.date.accessioned2014-05-20T15:20:22Z
dc.date.available2014-05-20T15:20:22Z
dc.date.issued2005-11-01
dc.description.abstractWe described a prophylactic and therapeutic effect of a DNA vaccine encoding the Mycobacterium leprae 65- kDa heat shock protein (DNA-hsp65) in experimental murine tuberculosis. However, high homology of the vaccine to the corresponding mammalian hsp60, together with the CpG motifs in the plasmidial vector, could trigger or exacerbate an autoimmune disease. In the present study, we evaluate the potential of DNA- hsp65 vaccination to induce or modulate arthritis in mice genetically selected for acute inflammatory reaction (AIR), either maximal (AIRmax) or minimal (AIRmin). Mice immunized with DNA-hsp65 or injected with the corresponding DNA vector (DNAv) developed no arthritis, whereas pristane injection resulted in arthritis in 62% of AIRmax mice and 7.3% of AIRmin mice. Administered after pristane, DNA- hsp65 downregulated arthritis induction in AIRmax animals. Levels of interleukin (IL)- 12 were significantly lower in mice receiving pristane plus DNA- hsp65 or DNAv than in mice receiving pristane alone. However, when mice previously injected with pristane were inoculated with DNA- hsp65 or DNAv, the protective effect was significantly correlated with lower IL-6 and IL-12 levels and higher IL-10 levels. Our results strongly suggest that DNA-hsp65 has no arthritogenic potential and is actually protective against experimentally induced arthritis in mice.en
dc.description.affiliationUniv Fed São Paulo, Dept Bioquim & Imunol, Ctr Pesquisa TB, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationInst Butantan, Lab Imunogenet, BR-05503900 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.format.extent1338-1345
dc.identifierhttp://dx.doi.org/10.1089/hum.2005.16.1338
dc.identifier.citationHuman Gene Therapy. New Rochelle: Mary Ann Liebert Inc., v. 16, n. 11, p. 1338-1345, 2005.
dc.identifier.doi10.1089/hum.2005.16.1338
dc.identifier.fileWOS000233346600012.pdf
dc.identifier.issn1043-0342
dc.identifier.lattes4977572416129527
dc.identifier.urihttp://hdl.handle.net/11449/31691
dc.identifier.wosWOS:000233346600012
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc.
dc.relation.ispartofHuman Gene Therapy
dc.relation.ispartofjcr4.241
dc.relation.ispartofsjr1,771
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleImmunomodulation and protection induced by DNA-hsp65 vaccination in an animal model of arthritisen
dc.typeArtigo
dcterms.licensehttp://www.liebertpub.com/nv/resources-tools/self-archiving-policy/51/
dcterms.rightsHolderMary Ann Liebert Inc.
dspace.entity.typePublication
unesp.author.lattes4977572416129527[2]
unesp.author.orcid0000-0002-0043-4568[9]
unesp.author.orcid0000-0003-4557-3331[2]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentMicrobiologia e Imunologia - IBBpt

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