Publicação: Gene expression during the germination of coffee seed
Carregando...
Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Abrates-assoc Brasileira Technologia Sementes
Tipo
Artigo
Direito de acesso
Acesso aberto
Resumo
Germination of the coffee (Coffea arabica L.) seed is the result of events that occur simultaneously in the embryo and endosperm. To understand the molecular mechanisms responsible for these events, we undertook a transcriptome analysis of embryo, micropylar and lateral endosperms from 10-day-imbibed seeds. The sequencing yielded contigs coding for 16,813 proteins. From those, 14,005 (similar to 83%) were highly similar to at least one protein sequence in the nr database. 162 genes were significantly expressed in the embryo, 36 in the micropylar endosperm and 72 in the lateral endosperm. The tissue specificity analysis of the significantly expressed genes showed that the embryo had the highest proportion of specific genes (113/162, similar to 70%), while 11 were expressed in the micropylar and lateral endosperms. In the embryo, genes were mainly associated with abiotic stress, cell growth, and intercellular communication. In the micropylar and lateral endosperms, they were associated with abiotic stress and cell wall degradation. The accuracy of RNA-seq data was confirmed by RT-qPCR. This work adds new information about the molecular mechanism involved in coffee seed germination.
Descrição
Palavras-chave
RNA-seq, embryo, micropylar endosperm, lateral endosperm, Coffea arabica
Idioma
Inglês
Como citar
Journal Of Seed Science. Londrina Pr: Abrates-assoc Brasileira Technologia Sementes, v. 41, n. 2, p. 168-179, 2019.
