Publicação: Indicator-free electrochemical DNA hybridization biosensor
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Elsevier B.V.
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Resumo
A new electrochemical hybridization biosensor protocol without an external indicator is described. The biosensor format involves the immobilization of inosine-substituted (guanine-free) probe onto the carbon paste transducer, and a direct chronopotentiometric detection of the duplex formation by the appearance of the guanine oxidation peak of the target. Such a use of the intrinsic DNA electrochemical response for monitoring hybridization events offers several advantages (over the common use of external indicators), including the appearance of a new peak, a Aat background, or simplicity. A 4 min short hybridization period allows a detection limit around 120 ng/ml. Performance characteristics of the sensor are described along with future prospects. (C) 1998 Elsevier B.V. B.V. All rights reserved.
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DNA hybridization, biosensors, guanine, inosine, carbon paste electrodes
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Inglês
Como citar
Analytica Chimica Acta. Amsterdam: Elsevier B.V., v. 375, n. 3, p. 197-203, 1998.
