Early career achievement award: Supplementing omega-6 fatty acids to enhance early embryonic development and pregnancy establishment in Bos indicus and B. taurus beef cows

Abstract

Our research group investigated the impacts of supplementing Ca salts of soybean oil (CSSO), a source of omega-6 fatty acids (FAs), on reproductive performance of beef cows. Initial studies were conducted with Nelore (Bos indicus) cows grazing tropical pastures. Cows were assigned to fixed-time artificial insemination (AI) and supplemented or not with 100 g/d (as-fed basis) of CSSO, and supplementation regimens ranged from days-11 to 28 relative to AI. Overall, CSSO supplementation during the 21 d after AI increased (P < 0.01) pregnancy rates from 38.1% (623/1,635 as pregnant/total nonsupplemented cows) to 49.0% (843/1,720 as pregnant/total CSSO-supplemented cows), and these outcomes were associated with enhanced early embryonic development and pregnancy establishment when omega-6 FA were supplemented. To verify this rationale, our group compared FA incorporation in grazing Nelore cows (n = 90) supplemented or not with CSSO (100 g/d; as-fed basis) beginning at fixed-time AI until slaughter at day 19 of gestation. Supplementing CSSO increased (P ≤ 0.05) incorporation of linoleic acid and its omega-6 derivatives in plasma, endometrium, corpus luteum, and conceptus, whereas the same responses were not observed (P ≥ 0.25) for omega-3 FA. Complementing these findings, grazing Nelore cows (n = 100) were supplemented or not with CSSO (100 g/d; as-fed basis) beginning at fixed-time AI, and assigned to transcervical uterine flush on day 15 of gestation. Supplementing CSSO increased (P ≤ 0.04) conceptus length (2.58 vs. 1.15 cm) and mRNA expression of interferon-tau (4.1-fold increase) and prostaglandin E synthase 2 (2.6-fold increase), which are critical regulators of pregnancy establishment. These outcomes were recently replicated in B. taurus beef cows consuming temperate forages. Pregnancy rates were greater (P = 0.01) in Angus cows receiving CSSO (100 g/d; as-fed basis) for 21 d after fixed-time AI (60.2%; 226/383 as pregnant/total cows) compared with nonsupplemented cows (51.7%; 193/388 as pregnant/total cows). Supplementing CSSO to Angus × Hereford cows (n = 96) beginning after AI also increased (P = 0.05) mRNA expression of interferon-tau in day 15 conceptuses (1.8-fold increase). Collectively, our research demonstrated that post-AI CSSO supplementation favors incorporation of omega-6 FA into maternal and embryonic tissues, which enhances interferon-tau synthesis by the conceptus and increases pregnancy rates to fixed-time AI in B. indicus and B. taurus beef cows.