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Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds

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dc.contributor.authorEvaristo, Thaiane Cristine
dc.contributor.authorCruzalves, Flávia Cilene Maciel Da
dc.contributor.authorMoroz, Andrei[UNESP]
dc.contributor.authorMion, Woner
dc.contributor.authorAcorci-valério, Michele Janegitz
dc.contributor.authorFelisbino, Sérgio Luis
dc.contributor.authorRossi-ferreira, Rosana
dc.contributor.authorRuiz Júnior, Raul Lopes [UNESP]
dc.contributor.authorDeffune, Elenice [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionHermínio Ometto University Center
dc.contributor.institutionPaulista University Department of Health Science
dc.date.accessioned2015-02-02T12:39:33Z
dc.date.available2015-02-02T12:39:33Z
dc.date.issued2014-08-01
dc.description.abstractPURPOSE:To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal.METHODS: This study was performed with New Zealand adult rabbits weighing 3.0 - 4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS and Triton X-100 detergents), and enzymatic methods (DNase and RNase).RESULTS: Generally, the cells surrounding soft tissues were successfully removed, but none protocol removed cells from the tracheal cartilage. However, longer protocols were more effective. The cost-benefits relation of the enzymatic processes was not favorable. It was possible to find out that the cartilaginous tissue submitted to the irradiation with LED 630nm and 475 nm showed an increased number of gaps without cells, but several cells were observed to be still present.CONCLUSION: The light-emitting diode is a promising tool for decellularization of soft tissues.en
dc.description.affiliationPaulista State University Medical School Department of Surgery and Orthopedy
dc.description.affiliationHermínio Ometto University Center
dc.description.affiliationUNESP School of Pharmaceutical Sciences Department of Bioprocess and Biotechnology
dc.description.affiliationUNESP Medical School Blood Transfusion Center
dc.description.affiliationPaulista University Department of Health Science
dc.description.affiliationUNESP Bioscience Institute Department of Morphology
dc.description.affiliationUNESP Faculty of Science Department of Science Biology
dc.description.affiliationUNESP Medical School Department of Surgery and Orthopedy
dc.description.affiliationUNESP Medical School Department of Urology
dc.description.affiliationUnespPaulista State University Medical School Department of Surgery and Orthopedy
dc.description.affiliationUnespUNESP School of Pharmaceutical Sciences Department of Bioprocess and Biotechnology
dc.description.affiliationUnespUNESP Medical School Blood Transfusion Center
dc.description.affiliationUnespUNESP Bioscience Institute Department of Morphology
dc.description.affiliationUnespUNESP Faculty of Science Department of Science Biology
dc.description.affiliationUnespUNESP Medical School Department of Surgery and Orthopedy
dc.description.affiliationUnespUNESP Medical School Department of Urology
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent485-492
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502014000800002
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 8, p. 485-492, 2014.
dc.identifier.doi10.1590/S0102-86502014000800002
dc.identifier.fileS0102-86502014000800485.pdf
dc.identifier.issn0102-8650
dc.identifier.lattes7566862391932816
dc.identifier.lattes9646764071339214
dc.identifier.scieloS0102-86502014000800485
dc.identifier.urihttp://hdl.handle.net/11449/114466
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.relation.ispartofjcr0.933
dc.relation.ispartofsjr0,395
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectTissue Engineeringen
dc.subjectBiocompatible Materialsen
dc.subjectQuantum Dotsen
dc.subjectTracheaen
dc.subjectRabbitsen
dc.titleLight-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffoldsen
dc.typeArtigo
dspace.entity.typePublication
unesp.author.lattes7566862391932816[7]
unesp.author.lattes9646764071339214
unesp.author.lattes6926124203948011[3]
unesp.author.orcid0000-0002-4498-9784[3]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina, Botucatupt
unesp.departmentCirurgia e Ortopedia - FMBpt
unesp.departmentUrologia - FMBpt

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Botucatu - FMB - Faculdade de Medicina