Probiotic fermented soy product stimulated cytokines in rats on a long-term carcinogenic model

Abstract

Lactic acid bacteria (LAB) when used as probiotics can promote stimulation of immune system through increase of the pathogen phagocytosis and also the cytokines production. The purpose of the present work was to evaluate cytokines production by peritoneal macrophages and splenic lynphocytes in rats with colon cancer induced 1,2 dimethylhydrazine (DMH) after treatment with soy product fermented with Enterococcus faecium CRL 183 supplemented with or without calcium. A total of 30 male Wistar SPF rats, randomly allocated to 6 groups (n=10), received the following treatments: Group 1- control; Group 2 -Positive control, rats with colon cancer induced with DMH; Group 3 -DMH rats treated with fermented soy product; Group 4- DMH rats treated with fermented soy and calcium; Group 5- rats treated with fermented soy alone; 6 Group- rats treated with fermented soy and calcium. The groups of induced animals received, subcutaneously, 20 mg/kg body weight of DMH in a weekly dose for 14 weeks. The non-induced animals were inoculated, subcutaneously, with 1mM EDTA (pH 6.5). At the end of the forty-second week the animals were euthanized in a CO 2chamber, Thioglycollate-elicited peritoneal exudates cells (PEC) were harvested from Wistar SPF rat using 5.0 mL of sterile phosphate-buffered saline (PBS), pH 7.4. The cells were washed twice by centrifugation at 200 g for 5 minutes at 4 °C and resuspended in appropriate medium for each test (H2O2, NO, TNF-α, IL-6, LFN-γ and LL-4). Results showed that in colon cancer animals, which were ingested fermented soy, product there is a higher immune system stimulation observed in increased of the H2O2 TNF- α, LFN-γ and LL4 production. Results suggest that ingestion of fermented soy product supplemented or not with calcium favor anti-tumoral response stimulating the cytokines produced by peritoneal macrophages and splenics lynphocytes. Copyright © 2011 by New Century Health Publishers, LLC.