Publicação:
Effect of hydrogen peroxide on thawed ovine sperm motility

dc.contributor.authorMaia, Marciane da Silva
dc.contributor.authorBicudo, Sony Dimas [UNESP]
dc.contributor.authorRodello, Leandro [UNESP]
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:56:48Z
dc.date.available2015-03-18T15:56:48Z
dc.date.issued2014-04-01
dc.description.abstractOxidative stress, resulting from excessive levels of ROS in semen, has a negative impact on functional parameters and sperm fertility. In this study we examined the influence of the oxidative stress induced by hydrogen peroxide (H2O2) in ovine sperm motility after thawing and catalase (CAT) ability to preserve sperm motility. Semen was incubated at 37 degrees C with 100 mu M H2O2, 1 U catalase + 100 mu M H2O2 or no treatment, for 30 min. Immediately after adding treatments, sperm motility was determined by computer-assisted semen analysis (CASA). Incubation with H2O2 led to a significant (P < 0.05) decrease in motility parameters whereas catalase prevented a decline in motility secondary to oxidative stress. After 30 min of incubation with H2O2, total motility (12.0% vs. control 73.0%, H2O2 +CAT 70.0%), progressive motility (0.0% vs. control 19.0%, H2O2 +CAT 19.0%) and rapid motility (1.0% vs. control 43.0%, H2O2 +CAT 40.0%) decreased significantly (P < 0.05), whereas percentage of static cells increased (84.0% vs. control 18.0%, H2O2 +CAT 20.0%). We conclude that H2O2 causes damage to ovine sperm motility and that catalase is able to avoid detrimental effect of H2O2 on sperm motility.en
dc.description.affiliationBrazilian Agr Res Corp EMBRAPA, Semiarid Res Ctr, Petrolina, PE, Brazil
dc.description.affiliationSao Paulo State Univ, Fac Vet Med & Anim Sci, Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Fac Vet Med & Anim Sci, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent119-123
dc.identifierhttp://www.cbra.org.br/pages/publicacoes/animalreproduction/issues/download/v11n2/pag119-123%20(AR654).pdf
dc.identifier.citationAnimal Reproduction. Belo Horizonte: Brazilian Coll Animal Reproduction, v. 11, n. 2, p. 119-123, 2014.
dc.identifier.fileWOS000346368100007.pdf
dc.identifier.issn1806-9614
dc.identifier.lattes8134606270913860
dc.identifier.urihttp://hdl.handle.net/11449/117701
dc.identifier.wosWOS:000346368100007
dc.language.isoeng
dc.publisherBrazilian Coll Animal Reproduction
dc.relation.ispartofAnimal Reproduction
dc.relation.ispartofjcr0.991
dc.relation.ispartofsjr0,308
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectantioxidantsen
dc.subjectcatalaseen
dc.subjectoxidative stressen
dc.subjectROSen
dc.titleEffect of hydrogen peroxide on thawed ovine sperm motilityen
dc.typeArtigo
dcterms.rightsHolderBrazilian Coll Animal Reproduction
dspace.entity.typePublication
unesp.author.lattes8134606270913860
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentReprodução Animal e Radiologia Veterinária - FMVZpt

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