Publicação: Effect of hydrogen peroxide on thawed ovine sperm motility
dc.contributor.author | Maia, Marciane da Silva | |
dc.contributor.author | Bicudo, Sony Dimas [UNESP] | |
dc.contributor.author | Rodello, Leandro [UNESP] | |
dc.contributor.institution | Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2015-03-18T15:56:48Z | |
dc.date.available | 2015-03-18T15:56:48Z | |
dc.date.issued | 2014-04-01 | |
dc.description.abstract | Oxidative stress, resulting from excessive levels of ROS in semen, has a negative impact on functional parameters and sperm fertility. In this study we examined the influence of the oxidative stress induced by hydrogen peroxide (H2O2) in ovine sperm motility after thawing and catalase (CAT) ability to preserve sperm motility. Semen was incubated at 37 degrees C with 100 mu M H2O2, 1 U catalase + 100 mu M H2O2 or no treatment, for 30 min. Immediately after adding treatments, sperm motility was determined by computer-assisted semen analysis (CASA). Incubation with H2O2 led to a significant (P < 0.05) decrease in motility parameters whereas catalase prevented a decline in motility secondary to oxidative stress. After 30 min of incubation with H2O2, total motility (12.0% vs. control 73.0%, H2O2 +CAT 70.0%), progressive motility (0.0% vs. control 19.0%, H2O2 +CAT 19.0%) and rapid motility (1.0% vs. control 43.0%, H2O2 +CAT 40.0%) decreased significantly (P < 0.05), whereas percentage of static cells increased (84.0% vs. control 18.0%, H2O2 +CAT 20.0%). We conclude that H2O2 causes damage to ovine sperm motility and that catalase is able to avoid detrimental effect of H2O2 on sperm motility. | en |
dc.description.affiliation | Brazilian Agr Res Corp EMBRAPA, Semiarid Res Ctr, Petrolina, PE, Brazil | |
dc.description.affiliation | Sao Paulo State Univ, Fac Vet Med & Anim Sci, Botucatu, SP, Brazil | |
dc.description.affiliationUnesp | Sao Paulo State Univ, Fac Vet Med & Anim Sci, Botucatu, SP, Brazil | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.format.extent | 119-123 | |
dc.identifier | http://www.cbra.org.br/pages/publicacoes/animalreproduction/issues/download/v11n2/pag119-123%20(AR654).pdf | |
dc.identifier.citation | Animal Reproduction. Belo Horizonte: Brazilian Coll Animal Reproduction, v. 11, n. 2, p. 119-123, 2014. | |
dc.identifier.file | WOS000346368100007.pdf | |
dc.identifier.issn | 1806-9614 | |
dc.identifier.lattes | 8134606270913860 | |
dc.identifier.uri | http://hdl.handle.net/11449/117701 | |
dc.identifier.wos | WOS:000346368100007 | |
dc.language.iso | eng | |
dc.publisher | Brazilian Coll Animal Reproduction | |
dc.relation.ispartof | Animal Reproduction | |
dc.relation.ispartofjcr | 0.991 | |
dc.relation.ispartofsjr | 0,308 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Web of Science | |
dc.subject | antioxidants | en |
dc.subject | catalase | en |
dc.subject | oxidative stress | en |
dc.subject | ROS | en |
dc.title | Effect of hydrogen peroxide on thawed ovine sperm motility | en |
dc.type | Artigo | |
dcterms.rightsHolder | Brazilian Coll Animal Reproduction | |
dspace.entity.type | Publication | |
unesp.author.lattes | 8134606270913860 | |
unesp.campus | Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatu | pt |
unesp.department | Reprodução Animal e Radiologia Veterinária - FMVZ | pt |
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