Logo do repositório
 

CDKN2A promoter hypermethylation in astrocytomas is associated with age and sex

Página do item simplificado
dc.contributor.authorAlves, Markênia Kélia Santos
dc.contributor.authorFaria, Mário Henrique Girão
dc.contributor.authorNeves Filho, Eduardo Henrique Cunha
dc.contributor.authorFerrasi, Adriana Camargo [UNESP]
dc.contributor.authorPardini, Maria Ines de Moura Campos [UNESP]
dc.contributor.authorMoraes Filho, Manoel Odorico de
dc.contributor.authorRabenhorst, Silvia Helena Barem
dc.contributor.institutionUniversidade Federal do Ceará (UFC)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:29:40Z
dc.date.available2014-05-27T11:29:40Z
dc.date.issued2013-06-12
dc.description.abstractCDKN2A promoter hypermethylation has been widely related to many cancers. In astrocytomas, although CDKN2A (p16INK4A protein) is often inactivated, there are still some controversial issues regarding the mechanism by which this alteration occurs. Thus, we analyzed a series of astrocytomas to assess the association between CDKN2A expression and methylation of grade I-IV tumors (WHO) and clinicopathological parameters. DNA extracted from formalin-fixed paraffin-embedded material of 93 astrocytic tumors was available for CDKN2A promoter methylation analysis and p16INK4A expression by methylation-specific PCR and immunohistochemistry, respectively. A strong negative correlation between nuclear and cytoplasmic immunostaining and CDKN2A promoter methylation was found. Additionally, a significant negative correlation between CDKN2A promoter methylation and age was observed; also, female patients had statistically more CDKN2A methylated promoters (p=0.036) than men. In conclusion, CDKN2A inactivation by promoter methylation is a frequent event in astrocytomas and it is related to the age and sex of patients. © 2013 Surgical Associates Ltd.en
dc.description.affiliationUniversidade Federal do Ceará Department of Pathology and Forensic Medicine, Rua Alexandre Baraúna, 949, Porangabussu, CEP 60183-630 Fortaleza
dc.description.affiliationUniversidade Estadual Paulista - UNESP Botucatu Medical School Molecular Biology Laboratory of Blood Transfusion Center, Distrito de Rubião Junior, s/n, CEP 18.618-000 Botucatu
dc.description.affiliationUniversidade Federal do Ceará Department of Physiology and Pharmacology, Rua Coronel Nunes de Melo, 1127, Porangabussu, CEP 60430-270 Fortaleza
dc.description.affiliationUnespUniversidade Estadual Paulista - UNESP Botucatu Medical School Molecular Biology Laboratory of Blood Transfusion Center, Distrito de Rubião Junior, s/n, CEP 18.618-000 Botucatu
dc.format.extent549-553
dc.identifierhttp://dx.doi.org/10.1016/j.ijsu.2013.05.030
dc.identifier.citationInternational Journal of Surgery, v. 11, n. 7, p. 549-553, 2013.
dc.identifier.doi10.1016/j.ijsu.2013.05.030
dc.identifier.issn1743-9191
dc.identifier.issn1743-9159
dc.identifier.lattes3587895085226224
dc.identifier.lattes4619588334582084
dc.identifier.orcid0000-0001-9200-5391
dc.identifier.scopus2-s2.0-84880329284
dc.identifier.scopus2-s2.0-84878686126
dc.identifier.urihttp://hdl.handle.net/11449/75638
dc.identifier.wosWOS:000321750800011
dc.language.isoeng
dc.relation.ispartofInternational Journal of Surgery
dc.relation.ispartofjcr2.693
dc.relation.ispartofsjr0,834
dc.relation.ispartofsjr0,834
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAge
dc.subjectAstrocytic tumors
dc.subjectCDKN2A Methylation
dc.subjectExpression
dc.subjectP16
dc.subjectcyclin dependent kinase inhibitor 2A
dc.subjectDNA
dc.subjectadolescent
dc.subjectadult
dc.subjectage
dc.subjectaged
dc.subjectastrocytoma
dc.subjectchild
dc.subjectDNA extraction
dc.subjectfemale
dc.subjectgene expression
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectpreschool child
dc.subjectpriority journal
dc.subjectpromoter region
dc.subjectprotein methylation
dc.subjectschool child
dc.subjectsex difference
dc.titleCDKN2A promoter hypermethylation in astrocytomas is associated with age and sexen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dspace.entity.typePublication
unesp.author.lattes3587895085226224[4]
unesp.author.lattes4619588334582084
unesp.author.orcid0000-0001-9200-5391[4]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina, Botucatupt
unesp.departmentClínica Médica - FMBpt

Arquivos

Coleções

Botucatu - FMB - Faculdade de Medicina