Araçatuba virus: A vaccinialike virus associated with infection in humans and cattle
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We describe a vaccinialike virus, Araçatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. Molecular characterization of the virus was done by using polymerase chain reaction amplification, cloning, and DNA sequencing of conserved orthopoxvirus genes such as the vaccinia growth factor (VGF), thymidine kinase (TK), and hemagglutinin. We used VGF-homologous and TK gene nucleotide sequences to construct a phylogenetic tree for comparison with other poxviruses. Gene sequences showed 99% homology with vaccinia virus genes and were clustered together with the isolated virus in the phylogenetic tree. Araçatuba virus is very similar to Cantagalo virus, showing the same signature deletion in the gene. Araçatuba virus could be a novel vaccinialike virus or could represent the spread of Cantagalo virus.
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animal cell Aracatuba virus cattle disease controlled study cowpox DNA sequence DNA virus gene amplification gene cluster gene deletion gene sequence molecular biology molecular cloning nonhuman nucleotide sequence Orthopoxvirus phylogeny polymerase chain reaction sequence analysis sequence homology Vaccinia virus Vero cell virogenesis virus gene virus identification virus infection virus isolation Animals Base Sequence Cattle Cattle Diseases Communicable Diseases, Emerging Genes, Viral Humans Microscopy, Electron Molecular Sequence Data Phylogeny Sequence Alignment Vaccinia Viral Proteins Viral Structural Proteins Viremia
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Emerging Infectious Diseases, v. 9, n. 2, p. 155-160, 2003.
