Risk assessment of 2β,3β-19α-trihydroxyursolic acid from Rubus imperialis (Rosaceae) in HepG2/C3A cells via genotoxicity, metabolism, and cell growth
| dc.contributor.author | Oshiiwa, Bruna [UNESP] | |
| dc.contributor.author | da Silva, Aline Pereira | |
| dc.contributor.author | Alves, Greice Rafaele | |
| dc.contributor.author | Filho, Valdir Cechinel | |
| dc.contributor.author | Niero, Rivaldo | |
| dc.contributor.author | O'Neill de Mascarenhas Gaivão, Isabel | |
| dc.contributor.author | de Oliveira, Liana Martins | |
| dc.contributor.author | de Lima, Luan Vitor Alves | |
| dc.contributor.author | Mantovani, Mário Sérgio | |
| dc.contributor.author | Maistro, Edson Luis [UNESP] | |
| dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
| dc.contributor.institution | Marilia Medical School (FAMEMA) | |
| dc.contributor.institution | Vale do Itajaí University (UNIVALI) | |
| dc.contributor.institution | University of Trás-os-Montes and Alto Douro | |
| dc.contributor.institution | Universidade Estadual de Londrina (UEL) | |
| dc.date.accessioned | 2025-04-29T18:50:17Z | |
| dc.date.issued | 2024-12-01 | |
| dc.description.abstract | Rubus imperialis (Rosaceae) is a Brazilian medicinal plant that already exhibited therapeutical perspectives. However, previous studies revealed cellular and/or genetic toxicity of extracts from aerial parts of this plant, as well as other species of the Rubus genus. Being 2β,3β-19α-trihydroxyursolic acid (2B) one of the major compounds of this plant, with proven pharmacological effect, it is important to investigate the biosafety of this isolated compound. Therefore, in the present study, (2B) was tested by several cytogenotoxic endpoints up to 20 μg/ml in human hepatoma HepG2/C3A cells. The test compound did not produce any decreased cell viability, DNA damage, chromosomal mutations, cell cycle changes, or apoptotic effects in the tested cells. Additionally, RT-qPCR analysis revealed the downregulation of CYP3A4 (metabolism), M-TOR (cell death), and CDKN1A (cell cycle) genes. Under the experimental conditions used, the 2B compound did not show cytogenotoxic activity after a single exposure to HepG2/C3A human cells. | en |
| dc.description.affiliation | Faculty of Philosophy and Sciences Speech and Hearing Therapy Department São Paulo State University (UNESP), São Paulo State | |
| dc.description.affiliation | Postgraduate Program in Health and Aging Marilia Medical School (FAMEMA), São Paulo State | |
| dc.description.affiliation | Programa de Pós-Graduação em Ciências Farmacêuticas e Núcleo de Investigações Químico-Farmacêuticas (NIQFAR) Vale do Itajaí University (UNIVALI), Santa Catarina State | |
| dc.description.affiliation | CECAV and Department of Genetics and Biotechnology University of Trás-os-Montes and Alto Douro | |
| dc.description.affiliation | Department of General Biology Londrina State University (UEL), Paraná State | |
| dc.description.affiliationUnesp | Faculty of Philosophy and Sciences Speech and Hearing Therapy Department São Paulo State University (UNESP), São Paulo State | |
| dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
| dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
| dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
| dc.description.sponsorshipId | FAPESP: 2017/24149-4 | |
| dc.description.sponsorshipId | CNPq: 303604/2021-2 | |
| dc.description.sponsorshipId | CAPES: Finance Code 001 | |
| dc.format.extent | 1886-1896 | |
| dc.identifier | http://dx.doi.org/10.1002/jat.4684 | |
| dc.identifier.citation | Journal of Applied Toxicology, v. 44, n. 12, p. 1886-1896, 2024. | |
| dc.identifier.doi | 10.1002/jat.4684 | |
| dc.identifier.issn | 1099-1263 | |
| dc.identifier.issn | 0260-437X | |
| dc.identifier.scopus | 2-s2.0-85200986959 | |
| dc.identifier.uri | https://hdl.handle.net/11449/300654 | |
| dc.language.iso | eng | |
| dc.relation.ispartof | Journal of Applied Toxicology | |
| dc.source | Scopus | |
| dc.subject | 2β,3β-19α-trihydroxyursolic acid toxicity | |
| dc.subject | apoptosis analysis | |
| dc.subject | cell cycle analysis | |
| dc.subject | comet assay | |
| dc.subject | gene expression | |
| dc.subject | micronucleus test | |
| dc.title | Risk assessment of 2β,3β-19α-trihydroxyursolic acid from Rubus imperialis (Rosaceae) in HepG2/C3A cells via genotoxicity, metabolism, and cell growth | en |
| dc.type | Artigo | pt |
| dspace.entity.type | Publication | |
| unesp.author.orcid | 0000-0001-5268-6508[9] | |
| unesp.campus | Universidade Estadual Paulista (UNESP), Faculdade de Filosofia e Ciências, Marília | pt |