Publicação: Bovine Herpesvirus 5 promotes mitochondrial dysfunction in cultured bovine monocyte-derived macrophages and not affect virus replication
Carregando...
Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Tipo
Artigo
Direito de acesso
Acesso aberto
Resumo
Bovine alphaherpesvirus 1 (BHV1) and 5 (BHV5) are known to establish latent infections in sensory neurons of the trigeminal ganglion, yet leukocytes and tonsils have also been described as sites of latency in experimentally infected cattle. Little information is available on which immune cells are susceptible to BHV5 infection and how the infection may affect cell bioenergy. The aim of this study was to determine whether primary bovine monocyte-derived macrophages are susceptible to BHV5 infection and to evaluate parameters such as cell survival, virus replication and nitric oxide (NO) production. In addition, production of reactive oxygen species and mitochondrial damage were also analyzed. BHV5 infected cells underwent low rates of apoptosis but activated mitochondrial membrane depolarization and complex I. Additionally, production of high NO levels upon monocyte derived-macrophage infection did not interfere with the production of progeny virus. Overall, our findings revealed that primary cultures of bovine monocyte-derived macrophages support BHV5 replication in vitro and that mitochondrial dysfunction induced by infection apparently does not interfere with virus replication.
Descrição
Palavras-chave
BHV5, Cell bioenergy, Innate immune cells, Mitochondrion
Idioma
Inglês
Como citar
Veterinary Microbiology, v. 229, p. 153-158.
