Fatores ambientais e fenológicos na atividade antimicrobiana e produção de ativos em Cochlospermum regium (Schrank) Pilg
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2013-12-09
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Universidade Estadual Paulista (Unesp)
Resumo
A espécie Cochlospermum regium foi selecionada por apresentar importantes propriedades terapêuticas relacionadas a infecções do sistema reprodutivo feminino, relatadas em levantamentos etnofarmacológicos. Entretanto, alguns estudos realizados com microorganismos in vitro são contraditórios. Este fato pode estar relacionado a fatores ambientais e fenológicos referentes ao cultivo da planta, a forma de preparação dos extratos e/ou também ao microrganismo alvo. No presente trabalho foram avaliadas a atividade antimicrobiana em raízes de plantas de C. regium coletadas em diversas localidades, estádios fenológicos, idade da planta e condições de sazonalidade. Para realização destes testes antimicrobianos foi desenvolvida uma metodologia a qual utiliza diretamente a droga vegetal sem a necessidade prévia de preparação de extratos (STAMP). Além disso, foram realizados estudos fitoquímicos biomonitorados através da bioautografia. Quanto ao STAMP, constatou-se que este é eficiente para avaliar drogas vegetais principalmente com atividade antifúngica. Com relação aos testes que avaliaram o efeito ambiental e fenológico sobre a atividade antimicrobiana de raízes de C. regium, foi verificado que, apesar de a planta apresentar atividade antimicrobiana em todas as condições avaliadas, foi possível observar que as raízes coletadas no outono e inverno apresentaram maior inibição frente a C. albicans, além disso, a idade da planta e substrato de cultivo, também interferem nesta resposta biológica. Desta maneira, para utilização de extratos desta planta contra essa doença infecciosa causada por C. albicans, recomenda-se a coleta de plantas no outono e inverno. Também foi possível observar que a entrecasca e cerne da raiz são os principais responsáveis pela atividade antimicrobiana. Os estudos fitoquímicos, realizados a partir do extrato hidroalcoólico (80%)...
Cochlospermum regium was selected because it has important therapeutic properties related to infections of female reproductive system, which is reported in ethnopharmacological studies. However, some studies with microorganisms in vitro using this plant are contradictory. This fact could be related to environmental or phenological factors relating to the cultivation of the plant, how to prepare the extracts and/or also the target organism. In this study has been evaluated the antimicrobial activity of C. regium roots collected in different localities, growth stages, plant age and seasonal conditions. To conduct these antimicrobials tests, a methodology has developed which uses the powered plant directly without prior preparation of extracts (STAMP). In addition, biomonitored phytochemical analysis were performed by bioautography assays. Regarding STAMP method, it was efficient for evaluating powered plant mainly with antifungal activity. The tests that evaluated the environmental and phenological effects on the antimicrobial activity of C. regium roots showed that despite the plant antimicrobial activity occur in all conditions evaluated, it has observed that the roots collected in autumn and winter showed greater inhibition against C. albicans. Furthermore, the age of the plant and the substrate of culture also affect the biological response. Thus, we recommend collecting plants in autumn and winter to use against infectious disease caused by C. albicans. It has also observed that both inner bark and root cern are the main responsible for the antimicrobial activity. The phytochemical studies, carried out from the hydroalcoholic extract (80%), showed that the active compounds are of medium polarity, since the highest activity has obtained in the ethyl acetate fraction. Gallic acid was found to be the majority compound 4 in this fraction from GC-MS. Two flavonoids have isolated in ...
Cochlospermum regium was selected because it has important therapeutic properties related to infections of female reproductive system, which is reported in ethnopharmacological studies. However, some studies with microorganisms in vitro using this plant are contradictory. This fact could be related to environmental or phenological factors relating to the cultivation of the plant, how to prepare the extracts and/or also the target organism. In this study has been evaluated the antimicrobial activity of C. regium roots collected in different localities, growth stages, plant age and seasonal conditions. To conduct these antimicrobials tests, a methodology has developed which uses the powered plant directly without prior preparation of extracts (STAMP). In addition, biomonitored phytochemical analysis were performed by bioautography assays. Regarding STAMP method, it was efficient for evaluating powered plant mainly with antifungal activity. The tests that evaluated the environmental and phenological effects on the antimicrobial activity of C. regium roots showed that despite the plant antimicrobial activity occur in all conditions evaluated, it has observed that the roots collected in autumn and winter showed greater inhibition against C. albicans. Furthermore, the age of the plant and the substrate of culture also affect the biological response. Thus, we recommend collecting plants in autumn and winter to use against infectious disease caused by C. albicans. It has also observed that both inner bark and root cern are the main responsible for the antimicrobial activity. The phytochemical studies, carried out from the hydroalcoholic extract (80%), showed that the active compounds are of medium polarity, since the highest activity has obtained in the ethyl acetate fraction. Gallic acid was found to be the majority compound 4 in this fraction from GC-MS. Two flavonoids have isolated in ...
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Plantas medicinais, Bioclimatologia, Agentes antiinfecciosos, Fenologia vegetal, Raizes - Cultivo, Plantas - Analise, Root-crops
Como citar
INÁCIO, Marielle Cascaes. Fatores ambientais e fenológicos na atividade antimicrobiana e produção de ativos em Cochlospermum regium (Schrank) Pilg. 2013. xiii, 94 f. Tese (doutorado)- Universidade Estadual Paulista, Júlio de Mesquita Filho, Faculdade de Ciências Agronômicas de Botucatu, 2013.