Rotura prematura de membranas pré-termo e corioamnionite histológica: aspectos da resposta imune inata e repercussões no período neonatal
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Data
2011-07-14
Autores
Polettini, Jossimara [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
A rotura prematura de membranas pré-termo (RPM-PT) é uma importante intercorrência obstétrica e a infecção da cavidade amniótica advinda do trato genital inferior é um dos principais fatores associados à sua fisiopatologia. As membranas corioamnióticas são barreiras mecânicas contra a ascensão de micro-organismos e possuem papel fundamental no sistema imune, pois são importantes fontes de mediadores inflamatórios como as citocinas e também de antimicrobianos naturais, como as defensinas. Em resposta à infecção, ocorrem recrutamento e ativação de leucócitos para as membranas fetais, o que caracteriza a corioamnionite histológica, que ativa a cascata inflamatória na interface materno-fetal e contribui com os mecanismos de enfraquecimento e rotura das membranas. Além acometer os tecidos gestacionais de gestações complicadas por RPM-PT, a corioamnionite histológica é um fator de risco para resultados adversos maternos e morbidades neonatais. 1) Quantificar a expressão de β defensinas (HBD1, 3 e 4) por membranas corioamnióticas de gestações complicadas por prematuridade associada à corioamnionite histológica; 2) Quantificar a expressão de RNA mensageiro (RNAm) e proteína de IL-18 em membranas corioamnióticas de mulheres com RPM-PT e correlacionar a expressão com a presença de corioamnionite histológica; 3) Avaliar os resultados neonatais adversos de gestações prétermo complicadas por corioamnionite histológica. Foram incluídas no estudo, gestantes com parto pré-termo e diagnóstico histológico de corioamnionite. Para o estudo da expressão de β defensinas, 40 fragmentos de membranas corioamnióticas, com diagnóstico histológico de corioamnionite, provenientes de gestações complicadas por rotura prematura de membranas pré-termo (RPM-PT) ou trabalho de parto prematuro com bolsa íntegra (TPP), que apresentaram parto...
The preterm premature rupture of membranes (PPROM) is an important obstetric issue, and infection in the amniotic cavity from the lower genital tract is one of the main factors associated with its physiology. Chorioamniotic membranes are mechanical barriers against the microorganism’s ascension, and they play a fundamental role in the immune system, since they are important sources of inflammatory mediators, such as cytokines, and of natural antimicrobials, like as defensins. In response to infection, leukocytes are recruited and activated in fetal membranes, which characterizes histological chorioamnionitis. This condition activates the inflammatory cascade on the maternal-fetal interface and contributes to weakening mechanisms and membrane rupture. In addition to affecting the gestational tissues of pregnancies complicated by PPROM, histological chorioamnionitis is a risk factor for adverse maternal outcomes and neonatal morbidities. 1) To quantify the expression of β defensins (HBD1, 3 and 4) by chorioamniotic membranes of pregnancies complicated by prematurity associated with histological chorioamnionitis; 2) To quantify the expression of IL-18 mRNA and protein in the chorioamniotic membranes of pregnant women with PPROM and correlate expression with histological chorioamnionitis; 3) To evaluate adverse neonatal outcomes in preterm pregnancies complicated by histological chorioamnionitis. Pregnant women with preterm delivery and histological diagnosis of chorioamnionitis were included in the study. In order to study the expression of β defensins, 40 fragments of chorioamniotic membranes with histological diagnosis of chorioamnionitis from pregnancies complicated by PPROM or preterm labor with intact membranes (PTL), and with preterm labor as a gestational outcome, comprised the study group. As a control group, 40 chorioamniotic membranes without chorioamnionitis and paired... (Complete abstract click electronic access below)
The preterm premature rupture of membranes (PPROM) is an important obstetric issue, and infection in the amniotic cavity from the lower genital tract is one of the main factors associated with its physiology. Chorioamniotic membranes are mechanical barriers against the microorganism’s ascension, and they play a fundamental role in the immune system, since they are important sources of inflammatory mediators, such as cytokines, and of natural antimicrobials, like as defensins. In response to infection, leukocytes are recruited and activated in fetal membranes, which characterizes histological chorioamnionitis. This condition activates the inflammatory cascade on the maternal-fetal interface and contributes to weakening mechanisms and membrane rupture. In addition to affecting the gestational tissues of pregnancies complicated by PPROM, histological chorioamnionitis is a risk factor for adverse maternal outcomes and neonatal morbidities. 1) To quantify the expression of β defensins (HBD1, 3 and 4) by chorioamniotic membranes of pregnancies complicated by prematurity associated with histological chorioamnionitis; 2) To quantify the expression of IL-18 mRNA and protein in the chorioamniotic membranes of pregnant women with PPROM and correlate expression with histological chorioamnionitis; 3) To evaluate adverse neonatal outcomes in preterm pregnancies complicated by histological chorioamnionitis. Pregnant women with preterm delivery and histological diagnosis of chorioamnionitis were included in the study. In order to study the expression of β defensins, 40 fragments of chorioamniotic membranes with histological diagnosis of chorioamnionitis from pregnancies complicated by PPROM or preterm labor with intact membranes (PTL), and with preterm labor as a gestational outcome, comprised the study group. As a control group, 40 chorioamniotic membranes without chorioamnionitis and paired... (Complete abstract click electronic access below)
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Palavras-chave
Emergencias neonatais, Neonatal emergencies
Como citar
POLETTINI, Jossimara. Rotura prematura de membranas pré-termo e corioamnionite histológica: aspectos da resposta imune inata e repercussões no período neonatal. 2011. 69 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Medicina de Botucatu, 2011.