Avaliação de escova ultrassônica em pacientes sob terapia ortodôntica. Estudo in vitro, clínico e microbiológico
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Data
2007-07-06
Autores
Costa, Mauricio Ribeiro [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O objetivo do estudo foi avaliar o efeito de uma escova ultrassônica, comparada a uma elétrica e a uma manual, sobre os parâmetros clínicos periodontais e microbiológicos de adolescentes sob terapia ortodôntica com aparelhos fixos. Paralelamente, foi avaliada a diminuição da força adesiva de braquetes ortodônticos, fixados a dentes humanos extraídos, após o uso de uma escova dentária ultrassônica comparada a uma escova elétrica. Material e Método: Para o experimento in vivo, 21 indivíduos (15,2l1,7) que utilizavam aparelhos fixos há mais de um ano foram divididos em três grupos de acordo com a seqüência de uso das escovas (crossover). Os pacientes usaram cada escova por um período de 30 dias, com intervalos de 15 dias entre as mesmas (washout). Amostras de saliva e placa bacteriana foram coletadas para realização de cultura microbiológica de Streptococcus grupo mutans e técnica de Checkerboard DNA-DNA Hybridization, respectivamente. No experimento in vitro, braquetes ortodônticos foram colados a superfície mais plana de quarenta e cinco dentes humanos extraídos. Posteriormente, os corpos de prova foram divididos em grupos de acordo com a escova a ser utilizada. Após o procedimento de escovação, a resistência adesiva foi avaliada em uma maquina de ensaios mecânicos. Resultado: Uma diferença significante no índice de placa (p<0,05) foi observada após o uso de escova ultrassônica nas superfícies vestibulares (10,32% pré e 3,96% pós-escovação). Da mesma forma, após o uso das escovas ultrassônicas e elétricas, as contagens de Streptococcus grupo mutans diminuíram significativamente (técnica de cultura microbiana - p<0,05). A técnica Checkerboard DNA-DNA hybridization apontou uma redução nos níveis de T. forsythia após o uso da escova elétrica e das espécies S. noxia, S. sanguinis...
This study compared the efficacy of an ultrasonic toothbrush for the reduction of plaque, gingival inflammation, and levels of bacteria in relation to an electric and a manual toothbrush. In addition, it was evaluated the effect of brushing with both an ultrasonic and electric toothbrush on the bond strength of orthodontic brackets bonded to extracted human teeth. Material and method: Twenty-one patients (mean 15.2l1.7 years) with orthodontic appliances were randomly divided into three groups according to the sequence of brush usage (crossover study). The subjects used each assigned brush for a period of 30 days, followed by an interval of 15 days (washout) during which they returned to their regular toothbrushes and dental floss used in accordance with the monthly instructions of the orthodontist given prior to the study. During each visit, participants received a periodontal evaluation and samples of saliva and subgingival plaque were collected for microbiological tests (Streptococcus mutans culture and DNA-DNA Checkerboard Hybridization). For in vitro evaluation, individual orthodontic brackets were bonded to the smoothest surface of forty-five teeth. The test specimens were randomly distributed into three groups according to the toothbrush to be used. After completing the brushing stage, the test specimens were taken to the mechanical assay machine for a shearing test. Results: The ultrasonic brush group presented significant improvement in the reduction of visible plaque on the buccal surfaces (10.32% against 3.96%). The counts of S. mutans decreased in the electric and ultrasonic groups (culture test - p<0.05). The DNA-DNA Checkerboard hybridization technique showed that the counts of T. forsythia decreased significantly after a month of electric brush usage. Furthermore, in the manual group, S. noxia, S. sanguinis and P. melaninogenica counts also decreased... (Complete abstract, click electronic access below)
This study compared the efficacy of an ultrasonic toothbrush for the reduction of plaque, gingival inflammation, and levels of bacteria in relation to an electric and a manual toothbrush. In addition, it was evaluated the effect of brushing with both an ultrasonic and electric toothbrush on the bond strength of orthodontic brackets bonded to extracted human teeth. Material and method: Twenty-one patients (mean 15.2l1.7 years) with orthodontic appliances were randomly divided into three groups according to the sequence of brush usage (crossover study). The subjects used each assigned brush for a period of 30 days, followed by an interval of 15 days (washout) during which they returned to their regular toothbrushes and dental floss used in accordance with the monthly instructions of the orthodontist given prior to the study. During each visit, participants received a periodontal evaluation and samples of saliva and subgingival plaque were collected for microbiological tests (Streptococcus mutans culture and DNA-DNA Checkerboard Hybridization). For in vitro evaluation, individual orthodontic brackets were bonded to the smoothest surface of forty-five teeth. The test specimens were randomly distributed into three groups according to the toothbrush to be used. After completing the brushing stage, the test specimens were taken to the mechanical assay machine for a shearing test. Results: The ultrasonic brush group presented significant improvement in the reduction of visible plaque on the buccal surfaces (10.32% against 3.96%). The counts of S. mutans decreased in the electric and ultrasonic groups (culture test - p<0.05). The DNA-DNA Checkerboard hybridization technique showed that the counts of T. forsythia decreased significantly after a month of electric brush usage. Furthermore, in the manual group, S. noxia, S. sanguinis and P. melaninogenica counts also decreased... (Complete abstract, click electronic access below)
Descrição
Palavras-chave
Gengivite, Aparelhos ortodonticos, Escovação dentária, Boca - Cuidado e higiene, Microbiologia, Gingivitis, Orthodontic appliances, Toothbrushing, Oral hygiene, Microbiology, Ultrasonic toothbrush
Como citar
COSTA, Mauricio Ribeiro. Avaliação de escova ultrassônica em pacientes sob terapia ortodôntica. Estudo in vitro, clínico e microbiológico. 2007. 182 p. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araraquara, 2007.