Efeitos dos fatores de crescimento fibroblástico 10 e 18 (FGFs 10 e 18) sobre a esteroidogênese em ovários fetais bovinos
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Data
2012-07-18
Autores
Silva, Rubia Bueno da [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Durante o desenvolvimento ovariano fetal, a formação folicular inicial é decisiva para a fertilidade da fêmea, pois define sua reserva gametogênica. Tem sido proposto que a progesterona e o estradiol desempenham papel regulatório na foliculogênese pré-antral, de forma que sua produção reduzida em ovários fetais bovinos antecede o surgimento de folículos primordiais e primários. Recentemente, os FGFs 10 e 18 foram reportados em folículos ovarianos bovinos como redutores dos níveis de esteróides, o que parece envolver a inibição da expressão de enzimas necessárias à esteroidogênese. Em adição, a expressão do FGF10 foi observada durante o desenvolvimento ovariano fetal bovino, e esteve positivamente associada ao aumento no número de folículos primários. O presente estudo investigou primeiramente o padrão de expressão do RNAm das enzimas esteroidogênicas (StAR, CYP11A1, 3β-HSD, CYP17A1, CYP19A1 e 17β-HSD) em ovários de fetos bovinos em idades gestacionais específicas (60, 75, 90, 120, 150 e 210 dias). Todos os genes investigados se mostraram expressos e regulados ao longo da gestação. Os níveis de RNAm da CYP19A1 diminuíram dos 60 para os 90 dias, sugerindo envolvimento desta enzima com a produção decrescente de estradiol observada previamente durante este período gestacional. A expressão das demais enzimas foi elevada ao longo da gestação, coincidente com o aumento da competência esteroidogênica descrito preliminarmente durante o desenvolvimento folicular inicial. Em adição, foi investigada a participação dos FGFs 10 e 18 na esteroidogênese ovariana fetal bovina. A expressão do FGF18 e de seus receptores (FGFR2C, FGFR3C e FGFR4) foi detectada em ovários fetais bovinos ao longo da gestação (60, 75, 90, 120, 150 e 210 dias). A abundância de RNAm do FGF18 aumentou...
During fetal ovarian development, early follicular formation is essential to female fertility, when the gametogenic reserve is defined. It has been proposed that progesterone and estradiol play regulatory role on preantral folliculogenesis, once its reduced production in bovine fetal ovaries precedes primordial and primary follicle assembly. Recentlly, FGFs 10 and 18 were reported in bovine ovarian follicles as reducers of steroids levels, and this seems to involve the inhibition of enzymes necessary to steroidogenesis. In addition, FGF10 expression was observed during bovine fetal ovary development, and it was positively associated with the elevation on primary follicles number. The present study first investigated the mRNA expression patterns for steroidogenic enzymes (StAR, CYP11A1, HSD3B1, CYP17A1, CYP19A1 and HSD17B1) in bovine fetal ovaries at specific gestational ages (60, 75, 90, 120, 150 e 210 days). Expression of all investigated genes was detected and regulated through gestation. Messenger RNA levels of CYP19A1 decreased from days 60 to 90 of gestation, suggesting involvement of this enzyme on decrescent estradiol production previously observed during this gestational period. The expression of other enzymes was elevated during gestational period, which was coincident with the enhance of steroidogenic competence previously described during early follicular development. In addition, the participation of FGFs 10 and 18 on steroidogenesis in bovine fetal ovaries was investigated. The expression of FGF18 and its receptors (FGFR2C, FGFR3C and FGFR4) was detected in bovine fetal ovaries through gestation (60, 75, 90, 120, 150 e 210 days). The mRNA abundance of FGF18 enhanced between 90 and 120 days and decreased at 210 days. The expression of FGFR2C and FGFR4 did not vary during... (Complete abstract click electronic access below)
During fetal ovarian development, early follicular formation is essential to female fertility, when the gametogenic reserve is defined. It has been proposed that progesterone and estradiol play regulatory role on preantral folliculogenesis, once its reduced production in bovine fetal ovaries precedes primordial and primary follicle assembly. Recentlly, FGFs 10 and 18 were reported in bovine ovarian follicles as reducers of steroids levels, and this seems to involve the inhibition of enzymes necessary to steroidogenesis. In addition, FGF10 expression was observed during bovine fetal ovary development, and it was positively associated with the elevation on primary follicles number. The present study first investigated the mRNA expression patterns for steroidogenic enzymes (StAR, CYP11A1, HSD3B1, CYP17A1, CYP19A1 and HSD17B1) in bovine fetal ovaries at specific gestational ages (60, 75, 90, 120, 150 e 210 days). Expression of all investigated genes was detected and regulated through gestation. Messenger RNA levels of CYP19A1 decreased from days 60 to 90 of gestation, suggesting involvement of this enzyme on decrescent estradiol production previously observed during this gestational period. The expression of other enzymes was elevated during gestational period, which was coincident with the enhance of steroidogenic competence previously described during early follicular development. In addition, the participation of FGFs 10 and 18 on steroidogenesis in bovine fetal ovaries was investigated. The expression of FGF18 and its receptors (FGFR2C, FGFR3C and FGFR4) was detected in bovine fetal ovaries through gestation (60, 75, 90, 120, 150 e 210 days). The mRNA abundance of FGF18 enhanced between 90 and 120 days and decreased at 210 days. The expression of FGFR2C and FGFR4 did not vary during... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Bovino, Esteroides, Enzimas, Ovarios, Fibroblasto - Crescimento, Fibroblasts
Como citar
SILVA, Rubia Bueno da. Efeitos dos fatores de crescimento fibroblástico 10 e 18 (FGFs 10 e 18) sobre a esteroidogênese em ovários fetais bovinos. 2012. 96 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária e Zootecnia, 2012.