Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene
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Data
2014-06-01
Autores
Soares, Vítor Yamashiro Rocha
Silva, Jailthon Carlos Da
Silva, Kleverton Ribeiro Da
Cruz, Maria Do Socorro Pires E
Santos, Marcos Pérsio Dantas
Ribolla, Paulo Eduardo Martins
Alonso, Diego Peres
Coelho, Luiz Felipe Leomil
Costa, Dorcas Lamounier
Costa, Carlos Henrique Nery
Título da Revista
ISSN da Revista
Título de Volume
Editor
Instituto Oswaldo Cruz, Ministério da Saúde
Resumo
An analysis of the dietary content of haematophagous insects can provide important information about the transmission networks of certain zoonoses. The present study evaluated the potential of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mitochondrial cytochrome B (cytb) gene to differentiate between vertebrate species that were identified as possible sources of sandfly meals. The complete cytb gene sequences of 11 vertebrate species available in the National Center for Biotechnology Information database were digested with Aci I, Alu I, Hae III and Rsa I restriction enzymes in silico using Restriction Mapper software. The cytb gene fragment (358 bp) was amplified from tissue samples of vertebrate species and the dietary contents of sandflies and digested with restriction enzymes. Vertebrate species presented a restriction fragment profile that differed from that of other species, with the exception of Canis familiaris and Cerdocyon thous. The 358 bp fragment was identified in 76 sandflies. Of these, 10 were evaluated using the restriction enzymes and the food sources were predicted for four: Homo sapiens (1), Bos taurus (1) and Equus caballus (2). Thus, the PCR-RFLP technique could be a potential method for identifying the food sources of arthropods. However, some points must be clarified regarding the applicability of the method, such as the extent of DNA degradation through intestinal digestion, the potential for multiple sources of blood meals and the need for greater knowledge regarding intraspecific variations in mtDNA.
Descrição
Palavras-chave
blood meal analysis, cytochrome B, PCR-RFLP
Como citar
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 109, n. 3, p. 379-383, 2014.