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dc.contributor.authorBaptista, A. A. S. [UNESP]
dc.contributor.authorDonato, T. C. [UNESP]
dc.contributor.authorGarcia, K. C. O. D. [UNESP]
dc.contributor.authorGoncalves, G. A. M. [UNESP]
dc.contributor.authorCoppola, M. P. [UNESP]
dc.contributor.authorOkamoto, Adriano Sakai [UNESP]
dc.contributor.authorSequeira, Julio Lopes [UNESP]
dc.contributor.authorAndreatti Filho, Raphael Lucio [UNESP]
dc.identifier.citationPoultry Science. Oxford: Oxford Univ Press, v. 93, n. 1, p. 39-45, 2014.
dc.description.abstractThis study investigated the immune response of broiler chickens with oral treatment of a Lactobacillus spp. pool (PL) associated with microencapsulated recombinant proteins flagellin (FliC) and the subunit B of cholera toxin (CTB). Immune responses were evaluated by measuring IgA from intestinal fluid, serum IgY, and immunostaining of CD8(+) T lymphocytes present in the cecum. The evaluations were performed on d 0, 7, 14, 21, and 28 posttreatment. A significant increase (P < 0.05) was observed in IgA levels in all immunized groups, especially 3 wk after immunization. Treatments 2 (recombinant CTB) and 3 (recombinant FliC+CTB) showed the highest concentrations. Similarly, serum concentrations IgY (mu g/mL) increased along the experiment, and the means for treatments 2 and 3 showed significant differences (P < 0.05) compared with controls, reaching concentrations of 533 and 540 mu g/mL, respectively. The number of CD8+ T lymphocytes in all treatments greatly differed (P < 0.05) compared with the negative control at 21 d posttreatment. However, only treatment 2 (recombinant CTB), 4 (PL), and 5 (recombinant FliC+ recombinant CTB + PL) remained significantly (P < 0.05) different from the control at 28 d posttreatment. Thus, it is concluded that the microencapsulated recombinant proteins administered orally to broiler chickens are capable of stimulating humoral and cellular immune response, and the combinations of these antigens with Lactobacillus spp. can influence the population of CD8+ T cells residing in the cecum.en
dc.description.sponsorshipSao Paulo State Foundation
dc.publisherOxford University Press
dc.relation.ispartofPoultry Science
dc.sourceWeb of Science
dc.subjectrecombinant flagellinen
dc.subjectrecombinant cholera toxin subunit Ben
dc.subjectLactobacillus speciesen
dc.subjectimmunoglobulin Aen
dc.subjectCD8(+) T lymphocyteen
dc.titleImmune response of broiler chickens immunized orally with the recombinant proteins flagellin and the subunit B of cholera toxin associated with Lactobacillus spp.en
dcterms.rightsHolderOxford Univ Press
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationSao Paulo State Univ, Coll Vet Med & Anim Sci, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Coll Vet Med & Anim Sci, BR-18618970 Botucatu, SP, Brazil
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdFAPESP: 09/53570-3
dc.description.sponsorshipIdFAPESP: 09/52980-3
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
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