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dc.contributor.authorSoares, Diana Gabriela de Souza [UNESP]
dc.contributor.authorMarcomini, Natália [UNESP]
dc.contributor.authorBasso, Fernanda Gonçalves [UNESP]
dc.contributor.authorPansani, Taisa Nogueira [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorCosta, Carlos Alberto de Souza [UNESP]
dc.date.accessioned2015-08-06T16:12:13Z
dc.date.available2015-08-06T16:12:13Z
dc.date.issued2015
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1111/iej.12426/abstract
dc.identifier.citationInternational Endodontic Journal, v. 48, p. 1-6, 2015.
dc.identifier.issn0143-2885
dc.identifier.urihttp://hdl.handle.net/11449/125485
dc.description.abstractAim To assess the initial cytotoxicity and the late phenotype marker expression of odontoblast-like cells (MDPC-23) subjected to less aggressive in-office bleaching therapies. Methodology A 17.5% hydrogen peroxide (H2O2) gel was applied for 45, 15 or 5 min to enamel/dentine discs adapted to trans-wells positioned over cultured MDPC-23 cells. No treatment was performed on the negative control. Immediately after bleaching, the cell viability, gene expression of inflammatory mediators and quantification of H2O2 diffusion were evaluated. The ALP activity, DSPP and DMP-1 gene expression and mineralized nodule deposition (MND) were assessed at 7, 14 or 21 days post-bleaching and analysed statistically with Mann–Whitney U-tests (α = 5%). Results H2O2 diffusion, proportional to treatment time, was observed in all bleached groups. Reductions of approximately 31%, 21% and 13% in cell viability were observed for the 45-, 15- and 5-min groups, respectively. This reduction was significant (P < 0.05) for the 45- and 15-min groups, which also presented significant (P < 0.05) over-expression of inflammatory mediators. The 45-min group was associated with significant (P < 0.05) reductions in DMP-1/DSPP expression at all periods, relative to control. The ALP activity and MND were reduced only in initial periods. The 15-min group had less intense reduction of all markers, with no difference to control at 21 days. Conclusions The 17.5% H2O2 applied to tooth specimens for 5 min caused no alteration in the odontoblast-like cells. When this gel was applied for 45 or 15 min, a slight cytotoxicity, associated with alterations in phenotypic markers, was observed. However, cells were able to recover their functions up to 21 days post-bleaching.en
dc.format.extent1-6
dc.language.isoeng
dc.relation.ispartofInternational Endodontic Journal
dc.sourceCurrículo Lattes
dc.subjectCell differentiationen
dc.subjectCytotoxicityen
dc.subjectOdontoblastsen
dc.subjectTooth bleachingen
dc.titleIndirect cytocompatibility of a low-concentrated hydrogen peroxide bleaching gel to odontoblast-like cellsen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Infantil, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801-903, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Infantil, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801-903, SP, Brasil
dc.description.affiliationUnespDepartment of Physiology and Pathology
dc.description.affiliationUnespDepartment of Orthodontics and Pediatric Dentistry
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics
dc.identifier.doi10.1111/iej.12426
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
dc.identifier.lattes8207097271172991
dc.identifier.lattes4517484241515548
unesp.departmentClínica Infantilpt
unesp.author.lattes8207097271172991
unesp.author.lattes4517484241515548
dc.relation.ispartofjcr3.015
dc.relation.ispartofsjr1,791
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