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dc.contributor.authorRodrigues, Jaqueline Polizeli
dc.contributor.authorFrei, Fernando
dc.contributor.authorNavarro, Italmar Teodorico
dc.contributor.authorSilva, Luciana Pereira
dc.contributor.authorMarcelino, Monica Yonashiro
dc.contributor.authorAndrade-Junior, Heitor Franco de
dc.contributor.authorFaria, Carolina Arruda de
dc.contributor.authorSantos, Marislene
dc.contributor.authorRibeiro-Paes, João Tadeu
dc.date.accessioned2015-08-21T17:54:46Z
dc.date.available2015-08-21T17:54:46Z
dc.date.issued2015
dc.identifierhttp://dx.doi.org/10.1186/1678-9199-21-1
dc.identifier.citationJournal of Venomous Animals and Toxins including Tropical Diseases. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 21, p. 1-7, 2015.
dc.identifier.issn1678-9199
dc.identifier.urihttp://hdl.handle.net/11449/127267
dc.description.abstractBackground Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet. Methods In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondiiantibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens. Results The IFAT method showed a seroprevalence of 22.3%. These results were similar to those obtained by ELISA (24.1%). The seroprevalence was directly estimated from the IgG avidity, which showed that in a sample of 112 students, three of them had acute infection, an incidence of 1.6% in the studied population. Conclusion In this study, the use of different chromogenic substrates in immunoenzymatic ELISA assays did not display different sensitivity in the detection of T. gondii-reagent serum. The extrapolation of results to this population must be carefully considered, since the investigation was conducted on a reduced sample. However, it allows us to emphasize the importance of careful and well prepared studies to identify risk factors for toxoplasmosis, to adopt preventive measures and to offer guidance to at-risk populations about the disease.en
dc.format.extent1-7
dc.language.isoeng
dc.publisherCentro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
dc.relation.ispartofJournal of Venomous Animals and Toxins including Tropical Diseases
dc.sourceSciELO
dc.subjectToxoplasma gondiien
dc.subjectToxoplasmosisen
dc.subjectEnzyme-linked immunosorbent assayen
dc.subjectIndirect fluorescent antibody testen
dc.subjectSeroprevalenceen
dc.subjectEpidemiologyen
dc.titleSeroepidemiological analysis of toxoplasmosis in college studentsen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionLondrina State University Department of Veterinary Medicine
dc.contributor.institutionUniversity of São Paulo Institute for Technological Research
dc.contributor.institutionUniversity of São Paulo Institute of Tropical Medicine of São Paulo
dc.contributor.institutionUniversity of São Paulo Ribeirão Preto Medical School Department of Genetics
dc.description.affiliationUNESP Department of Biological Sciences
dc.description.affiliationLondrina State University Department of Veterinary Medicine
dc.description.affiliationUniversity of São Paulo Institute for Technological Research
dc.description.affiliationUniversity of São Paulo Institute of Tropical Medicine of São Paulo
dc.description.affiliationUniversity of São Paulo Ribeirão Preto Medical School Department of Genetics
dc.description.affiliationUniversidade Estadual Paulista Laboratório de Genética e Terapia Celular Departamento de Ciências Biológicas
dc.description.affiliationUnespUNESP Department of Biological Sciences
dc.description.affiliationUnespUniversidade Estadual Paulista Laboratório de Genética e Terapia Celular Departamento de Ciências Biológicas
dc.identifier.doi10.1186/1678-9199-21-1
dc.identifier.scieloS1678-91992015000100311
dc.rights.accessRightsAcesso aberto
dc.identifier.fileS1678-91992015000100311.pdf
unesp.author.orcid0000-0002-3354-8430[2]
dc.relation.ispartofjcr1.782
dc.relation.ispartofsjr0,573
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