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dc.contributor.authorFerrari, Sarita Priscila Gobbo [UNESP]
dc.contributor.authorBonassa, Karina Parra Duarte
dc.contributor.authorCoelho, Mirela Batista
dc.contributor.authorFerreira, Christina Ramires
dc.contributor.authorCosta, Heni Falcao da [UNESP]
dc.contributor.authorJara, Jose Luis Paz
dc.contributor.authorMiguel, Maria Carolina Villani [UNESP]
dc.contributor.authorReyes, Felix Guillermo Reyes
dc.contributor.authorEberlin, Marcos Nogueira
dc.contributor.authorNogueira, Guilherme de Paula [UNESP]
dc.contributor.authorSimas, Rosineide Costa [UNESP]
dc.date.accessioned2015-10-21T13:10:03Z
dc.date.available2015-10-21T13:10:03Z
dc.date.issued2015-01-01
dc.identifierhttp://pubs.rsc.org/en/Content/ArticleLanding/2015/AY/C4AY02962C#!divAbstract
dc.identifier.citationAnalytical Methods. Cambridge: Royal Soc Chemistry, v. 7, n. 7, p. 3291-3297, 2015.
dc.identifier.issn1759-9660
dc.identifier.urihttp://hdl.handle.net/11449/128460
dc.description.abstractFluoroquinolones (FQs) are synthetic antimicrobials commonly used in intensive poultry farming to treat chronic respiratory disease, colibacillosis, and fowl cholera. As these drugs are also important in treating infections in humans, their use in poultry has been restricted in some countries to avoid the development of antimicrobial-resistant bacteria. In this work, a novel simple and efficient LC-MS/MS method using solid phase extraction (SPE), ESI ionization and multiple reaction mode (MRM) has been developed and validated to determine the two most common FQ residues: enrofloxacin (ENR) and ciprofloxacin (CIP) in chicken target tissues (muscle, liver, kidney, fat and skin) and in plasma. The method is shown to be sensitive, with limits of quantification (LOQ) between 1 ng g(-1) and 5 ng g(-1), recoveries 93-115% and correlation coefficients greater than 0.99. This novel methodology allows faster and simpler sample workflow compared to previously reported methodologies, making it ideal for high-throughput screening and confirmation of both ENR and CIP meeting the international regulation levels.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipInternational Atomic Energy Agency
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent3291-3297
dc.language.isoeng
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofAnalytical Methods
dc.sourceWeb of Science
dc.titleHigh precision and selectivity for quantitation of enrofloxacin and ciprofloxacin in five chicken tissues using solid phase extraction and ESI LC-MS/MS for application in monitoring residuesen
dc.typeArtigo
dcterms.licensehttp://www.rsc.org/journals-books-databases/open-access/
dcterms.rightsHolderRoyal Soc Chemistry
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.description.affiliationUniversidade Estadual de Campinas, Departamento de Ciência de Alimentos, Faculdade de Engenharia de Alimentos
dc.description.affiliationUniversidade Estadual de Campinas, Instituto de Química
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Apoio, Produção e Saúde Animal, Faculdade de Medicina Veterinária de Araçatuba
dc.identifier.doi10.1039/c4ay02962c
dc.identifier.wosWOS:000351931000051
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdFAPESP: 2010/51677-2
dc.description.sponsorshipIdFAPESP: 2010/15167-0
dc.description.sponsorshipIdInternational Atomic Energy Agency: CRP D5.20.36
dc.description.sponsorshipIdCAPES: 02500/094
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatubapt
dc.identifier.lattes6926188473133379
unesp.author.lattes6926188473133379
dc.relation.ispartofjcr2.073
dc.relation.ispartofsjr0,619
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