An impedimetric biosensor to test neat serum for dengue diagnosis

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Data

2015-07-05

Autores

Cecchetto, Juliana [UNESP]
Carvalho, Fernanda C. [UNESP]
Santos, Adriano [UNESP]
Fernandes, Flavio C. B. [UNESP]
Bueno, Paulo R. [UNESP]

Título da Revista

ISSN da Revista

Título de Volume

Editor

Elsevier B.V.

Resumo

NS1, a non-structural dengue protein, has been used as a biomarker for the detection of viremia in dengue diagnosis. In this work, an impedimetric label-free immunosensor based on an anti-NS1 modified gold electrode was developed. To immobilize anti-NS1, a mixed self-assembled monolayer consisting of 11-mercaptoundecanoic acid (for covalent anti-NS1 attachment) and 6-mercaptohexanol (as a spacer) was prepared. The impedance spectra were recorded in the presence of a redox probe ( [Fe(CN)(6)](3-/4-)) to monitor changes in charge-transfer resistance associated with target binding both in PBS and neat serum. A calibration curve was constructed in order to obtain analytical parameters. NS1 was diluted in PBS to final concentrations in the linear range of 0.01-2.00 mu g mL(-1) with a sensitivity of 14.1 percentage decade(-1) (R similar to 0.99) and a limit of detection (LOD) of 3 ng mL(-1). A linear range 0.01-1.00 mu g mL(-1) of NS1 diluted in neat serum was also obtained (R similar to 0.98) with a sensitivity of 10.4 percentage decade(-1) and a LOD of 30 ng mL(-1). The results open new insights into the potential use of this biosensor for point-of-care and bedside applications for dengue diagnosis.

Descrição

Palavras-chave

Dengue, NS1 protein, Electrochemical impedance spectroscopy (EIS), Label-free biosensor, Blood serum

Como citar

Sensors And Actuators B-chemical. Lausanne: Elsevier Science Sa, v. 213, p. 150-154, 2015.