beta-Glucan extracted from the medicinal mushroom Agaricus blazei prevents the genotoxic effects of benzo[a]pyrene in the human hepatoma cell line HepG2
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Data
2009-01-01
Autores
Friedmann Angeli, Jose Pedro
Ribeiro, Lúcia Regina [UNESP]
Bellini, Marilanda Ferreira
Mantovani, Mario Sergio
Título da Revista
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Título de Volume
Editor
Springer
Resumo
The mushroom Agaricus blazei is studied for its nutraceutical potential and as a medicinal supplement. The aim of the present study was to investigate the chemoprotective effect of beta-glucan extracted from the mushroom A. blazei against DNA damage induced by benzo[a]pyrene (B[a]P), using the comet assay (genotoxicity) and micronucleus assay with cytokinesis block (mutagenicity) in a human hepatoma cell line (HepG2). To elucidate the possible beta-glucan mechanism of action, desmutagenesis or bioantimutagenesis types, three treatment protocols were tested: simultaneous, pre-treatment, and presimultaneous. The results showed that beta-glucan does not exert genotoxic or mutagenic effect, but that it does protect against DNA damage caused by B[a]P in every protocol tested. The data suggest that beta-glucan acts through binding to B[a]P or the capture of free radicals produced during its activation. on the other hand, the pre-treatment results also suggest the possibility that beta-glucan modulates cell metabolism.
Descrição
Palavras-chave
beta-Glucan, Comet assay, Micronucleus assay, HepG2, Antigenotoxicity, Antimutagenicity
Como citar
Archives of Toxicology. New York: Springer, v. 83, n. 1, p. 81-86, 2009.