Coinfec��o com Hepatozoon canis e Leishmania spp. Em c�es diagnosticados com leishmaniose visceral
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Data
2016-01-01
Autores
Morgado, Fernanda Nazar�
Cavalcanti, Amanda dos Santos
de Miranda, Luisa Helena
O’Dwyer, L�cia Helena [UNESP]
da Silva, Maria Regina Lucas [UNESP]
Menezes, Rodrigo Caldas
da Silva, Aurea Virg�nia Andrade
Boit�, Mariana C�rtes
Cupolillo, Elisa
Porrozzi, Renato
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Resumo
This study describes the occurrence of dogs naturally co-infected with Hepatozoon canis and two Leishmania species: L. infantum or L. braziliensis. Four dogs serologically diagnosed with Visceral Leishmaniasis were euthanized. Liver and spleen samples were collected for histopathological analysis and DNA isolation. H. canis meronts were observed in tissues from all four dogs. H. canis infection was confirmed by PCR followed by sequencing of a fragment of 18S rRNA gene. Leishmania detection and typing was confirmed by ITS1’ PCR-RFLP and parasite burden was calculated using ssrRNA quantitative qPCR. A DPP-Dual Path platform test was performed. One out (Dog #2) of four animals was asymptomatic. Dogs #1 and #4 were infected by L. infantum and were DPP test positive. Dogs #2 and #3 were infected by L. braziliensis and were DPP test negative. Furthermore, visceral dissemination was observed in Dogs #2 and #3, since L. braziliensis was detected in liver and spleen samples. The visceral dissemination of L. braziliensis associated with systemic signs suggested that this co-infection could influence the parasite burden and disease progression.
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Canine visceral leishmaniasis, Co-infection, Hepatozoon canis, Hepatozoonosis, Leishmania braziliensis, Leishmania infantum
Como citar
Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 4, p. 450-458, 2016.