Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms
Carregando...
Arquivos
Data
2017-10-21
Autores
Castillo Pedraza, Midian C. [UNESP]
Novais, Tatiana F. [UNESP]
Faustoferri, Roberta C.
Quivey, Robert G.
Terekhov, Anton
Hamaker, Bruce R.
Klein, Marlise I. [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Streptococcus mutans-derived exopolysaccharides are virulence determinants in the matrix of biofilms that cause caries. Extracellular DNA (eDNA) and lipoteichoic acid (LTA) are found in cariogenic biofilms, but their functions are unclear. Therefore, strains of S. mutans carrying single deletions that would modulate matrix components were used: eDNA–∆lytS and ∆lytT; LTA–∆dltA and ∆dltD; and insoluble exopolysaccharide–ΔgtfB. Single-species (parental strain S. mutans UA159 or individual mutant strains) and mixed-species (UA159 or mutant strain, Actinomyces naeslundii and Streptococcus gordonii) biofilms were evaluated. Distinct amounts of matrix components were detected, depending on the inactivated gene. eDNA was found to be cooperative with exopolysaccharide in early phases, while LTA played a larger role in the later phases of biofilm development. The architecture of mutant strains biofilms was distinct (vs UA159), demonstrating that eDNA and LTA influence exopolysaccharide distribution and microcolony organization. Thus, eDNA and LTA may shape exopolysaccharide structure, affecting strategies for controlling pathogenic biofilms.
Descrição
Palavras-chave
Biofilms, eDNA, exopolysaccharides, extracellular matrix, lipoteichoic acids, Streptococcus mutans
Como citar
Biofouling, v. 33, n. 9, p. 722-740, 2017.