Antihypertensive therapy in pre-eclampsia: Effects of plasma from nonresponsive patients on endothelial gene expression
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Data
2016-07-01
Autores
Luizon, Marcelo R. [UNESP]
Caldeira-Dias, Mayara [UNESP]
Deffune, Elenice [UNESP]
Fernandes, Karla S.
Cavalli, Ricardo C.
Tanus-Santos, José E.
Sandrim, Valéria C. [UNESP]
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Resumo
Aim: Over 40% of patients with pre-eclampsia are nonresponsive to antihypertensive therapy, but the underlying mechanisms are unknown. We examined the effects of plasma from nonresponsive and responsive patients on endothelial gene expression. Patients & methods: PCR array was performed in human umbilical vein endothelial cells (HUVEC) incubated with plasma from nonresponsive (n = 4) and responsive (n = 4) patients. Gene networks and interactions with antihypertensive drugs used in pre-eclampsia were identified by Ingenuity Pathway Analysis. Results: Nifedipine and hydralazine act by upregulate or downregulate genes found to be downregulated or upregulated in HUVEC incubated with plasma from nonresponsive patients. Conclusion: Our novel findings suggest that plasma from nonresponsive and responsive patients evoke different responses in HUVEC, and might advance the pharmacogenomics research in pre-eclampsia.
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antihypertensive drugs, gene expression, human umbilical vein endothelial cells, hydralazine, nifedipine, pathway analysis, PCR array, pharmacogenomics, pre-eclampsia
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Pharmacogenomics, v. 17, n. 10, p. 1121-1127, 2016.