Mechanisms of action underlying the gastric antiulcer activity of the Rhizophora mangle L.
Carregando...
Arquivos
Data
2012-01-06
Autores
de-Faria, Felipe Meira
Alves Almeida, Ana Cristina
Luiz-Ferreira, Anderson
Dunder, Ricardo Jose
Takayama, Christiane
da Silva, Maria Silene
da Silva, Marcelo Aparecido [UNESP]
Vilegas, Wagner [UNESP]
Rozza, Ariane Leite [UNESP]
Pellizzon, Claudia Helena [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
Ethnopharmacological relevance: Rhizophora mangle, the red mangrove, has long been known as a traditional antiulcer medicine. The present work evaluated the mechanisms of action involved in the anti-ulcer properties of the Rhizophora mangle bark extracts.Materials and methods: Gastroprotection of Rhizophora mangle was evaluated in rodent experimental models (ethanol). To elucidate the mechanisms of action the antisecretory action and involvement of NO, SH, mucus and PGE(2) were evaluated. The acetic acid-induced gastric ulcer model. Western blotting assay (COX-1, COX-2 and EGF) and immunohistochemical localization of HSP-70, PCNA and COX-2 were also used to evaluate the Rhizophora mangle healing properties.Results: Results showed that Rhizophora mangle bark crude extract (CE), as well as ethyl acetate (EtOAc) and butanolic fractions (BuOH) provided significant gastroprotection at all the tested doses. Thereby, the following protocols were performed using the lowest dose capable of producing the most effective gastroprotection, which was the BuOH 0.5 mg/kg (P<0.001). Several mechanisms are involved in the antiulcer activity of Rhizophora mangle, such as, participation of NO, SH and mucus. The enhancement of PGE2 levels and the upregulation of COX-2 and EGF seem to be directly linked to the antisecretory, cytoprotective and healing effects of BuOH. HSP-70 and PCNA are also involved in this cicatrisation process. No sign of toxicity was observed in this study, considering the analyzed parameters.Conclusion: Our study reinforces its traditional medicinal use. Considering that the current therapies are based on the use of antisecretory or cytoprotective drugs, the Rhizophora mangle arises as a promising alternative antiulcer therapy. (C) 2011 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
Rhizophora mangle, Red mangrove, Tannin, Gastroprotection, Healing, Prostaglandins
Como citar
Journal of Ethnopharmacology. Clare: Elsevier B.V., v. 139, n. 1, p. 234-243, 2012.