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dc.contributor.authorSakamoto, Isabel Kimiko
dc.contributor.authorMaintiguer, Sandra Imaculada [UNESP]
dc.contributor.authorAmancio Varesche, Maria Bernadete
dc.date.accessioned2020-12-10T17:00:14Z
dc.date.available2020-12-10T17:00:14Z
dc.date.issued2019-01-01
dc.identifierhttp://dx.doi.org/10.4025/actascitechnol.v41i1.39128
dc.identifier.citationActa Scientiarum-technology. Maringa: Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao, v. 41, 11 p., 2019.
dc.identifier.issn1806-2563
dc.identifier.urihttp://hdl.handle.net/11449/194969
dc.description.abstractGranulated sludge from anaerobic reactors is constituted by the microbial consortia responsible for the degradation of different substrate present in wastewaters. This study characterized anaerobic microorganisms in a granular sludge from a Uasb reactor (Upflow Anaerobic Sludge Blanket) by Most Probable Number (MPN) technique and method of cloning and sequencing the 16S rDNA gene. The main objective of this study was to quantify and to identify the microorganisms in two different culture media containing sulfate (Posgate C [6.0 sodium lactate and 3.13 g L-1 sodium sulfate] and Zinder [2.24 sodium lactate and 0.96 g L-1 sodium sulfate]). Microorganisms quantified by Postgate C and Zinder media were: 9.30x10(10) and 7.50x10(11) MPN mL(-1) for general anaerobic bacteria; 4.30x10(8) and 2.10x10(8) MPN mL(-1) for sulfate reducing bacteria; and 1.20x10(8) and 1.5x10(8) MPN mL(-1) for methane producing archaea, respectively. Bacteria identified by 16S rDNA gene sequencing from the MPN in both culture media were related to the phyla Firmicutes and Proteobacteria. The conditions of Postgate C culture medium favored the sulfate-reducing bacteria and the Zinder culture medium favored the general anaerobic bacteria.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipBrazilian Innovation Agency (Finep)
dc.format.extent11
dc.language.isoeng
dc.publisherUniv Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao
dc.relation.ispartofActa Scientiarum-technology
dc.sourceWeb of Science
dc.subjectsulfate reducing bacteria
dc.subjectmethane producing archaea
dc.subject16S rDNA gene
dc.subjectcloning/sequencing
dc.subjectupflow anaerobic sludge blanket reactor
dc.titlePhylogenetic characterization and quantification by Most Probable Number of the microbial communities of biomass from the Upflow Anaerobic Sludge Blanket Reactor under sulfidogenic conditionsen
dc.typeArtigo
dcterms.rightsHolderUniv Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationUniv Sao Paulo, Dept Hidraul & Saneamento, Escola Engn Sao Carlos, Ave Joao Dagnone 1100, BR-13563120 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Pesquisa Bioenergia, Rio Claro, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Pesquisa Bioenergia, Rio Claro, SP, Brazil
dc.identifier.doi10.4025/actascitechnol.v41i1.39128
dc.identifier.wosWOS:000496531700034
unesp.author.orcid0000-0003-3124-7471[3]
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